Detection and quantification of Leishmania braziliensis in ectoparasites from dogs

dc.creatorMorais, Rayana Carla Silva de
dc.creatorGonçalves-de-Albuquerque, Suênia da Cunha
dc.creatorPessoa e Silva, Rômulo
dc.creatorCosta, Pietra Lemos
dc.creatorSilva, Kamila Gaudêncio da
dc.creatorSilva, Fernando José da
dc.creatorBrandão-Filho, Sinval Pinto
dc.creatorDantas-Torres, Filipe
dc.creatorPaiva-Cavalcanti, Milena de
dc.date2017-12-14T11:31:34Z
dc.date2017-12-14T11:31:34Z
dc.date2013
dc.date.accessioned2023-09-26T21:33:24Z
dc.date.available2023-09-26T21:33:24Z
dc.identifierMORAIS, R. C. S. de et al. Detection and quantification of Leishmania braziliensis in ectoparasites from dogs. Veterinary Parasitology, v. 196, n. 3–4, p. 506–508, 23 set. 2013.
dc.identifier1873-2550
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23654
dc.identifier10.1016/j.vetpar.2013.03.026
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872803
dc.descriptionAmerican cutaneous leishmaniasis (ACL) is a disease caused by different species of Leishmania protozoa, Leishmania braziliensis being the main species found in Brazil. In this study, two rural areas in Pernambuco, northeastern Brazil, where ACL is endemic, were selected. Genomic DNA was extracted from canine ectoparasites (ticks, fleas, and lice) and tested using a conventional PCR and a quantitative real time PCR. A total of 117 ectoparasites were collected, being 50 (42.74%) of them positive for L. braziliensis (in at least one PCR protocol), with a mean parasite load of 14.14 fg/μL. Furthermore, 46 (92.00%) positive ectoparasites were collected from positive dogs and 4 (8.00%) from negative ones. This study reports the detection of L. braziliensis DNA in ectoparasites, but does not prove their vector competence. Certainly, experimental transmission studies are necessary to assess their role, if any, in the transmission of Leishmania parasites to dogs.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectEctoparasitas
dc.subjectCães
dc.subjectLeishmania braziliensis
dc.subjectPCR
dc.subjectPCR em tempo real
dc.subjectEctoparasites
dc.subjectDogs
dc.subjectLeishmania braziliensis
dc.subjectPCR
dc.subjectReal time PCR
dc.subjectDoenças do Cão
dc.subjectparasitologia
dc.subjectLeishmania braziliensis
dc.subjectisolamento & purificação
dc.subjectFtirápteros
dc.subjectparasitologia
dc.subjectSifonápteros
dc.subjectparasitologia
dc.subjectCarrapatos
dc.subjectparasitologia
dc.subjectAnimais
dc.subjectCães
dc.subjectDNA de Protozoário
dc.subjectisolamento & purificação
dc.subjectEctoparasitoses
dc.subjectparasitologia
dc.subjectEctoparasitoses
dc.subjectveterinária
dc.titleDetection and quantification of Leishmania braziliensis in ectoparasites from dogs
dc.typeArticle


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