Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis

dc.creatorNunes, Marise P.
dc.creatorSobral, Ana Cristina L.
dc.creatorCoutinho, Sérgio G.
dc.date2020-09-20T20:46:47Z
dc.date2020-09-20T20:46:47Z
dc.date1992
dc.date.accessioned2023-09-26T21:33:15Z
dc.date.available2023-09-26T21:33:15Z
dc.identifierNUNES, Marise P.; SOBRAL, Ana Cristina L.; COUTINHO, Sérgio G. Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 503-509, Oct./Dec.1992.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43489
dc.identifier10.1590/S0074-02761992000400007
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872795
dc.descriptionLimiting dilution analysis was used to quantify Trypanosoma cruzi in the lymph nodes, liver and heart of Swiss and C57 B1/10 mice. The results showed that, in Swiss and B1/10 mice infected with T. cruzi Y strain, the number of parasites/mg of tissue increased during the course of the infection in both types of mice, although a grater number of parasites were observed in heart tissue from Swiss mice than from B1/10. With regard to liver tissue, it was observed that the parasite load in the initial phase of infection was higher than in heart. In experiments using T. cruzi Colombian strain, the parasite load in the heart of Swiss and B1/10 mice increased relatively slowly, although high levels of parasitization were nonetheless observable by the end of the infection. As for the liver and lymph nodes, the concentration of parasites was lower over the entire course of infection than in heart. Both strains thus maintained their characteristic tissue tropisms. The limiting dilution assay (LDA) proved to be an appropriate method for more precise quantification of T. cruzi, comparing favorably with other direct microscopic methods that only give approximate scores.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectInfecção experimental
dc.subjectLimitando a análise de diluição
dc.subjectTrypanosoma cruzi
dc.subjectLimiting dilution analysis
dc.subjectExperimental infection
dc.titleQuantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
dc.typeArticle


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