The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis

dc.creatorMörking, Patricia Alves
dc.creatorRampazzo, Rita de Cássia Pontello
dc.creatorWalrad, Pegine
dc.creatorProbst, Christian Macagnan
dc.creatorSoares, Maurilio José
dc.creatorGradia, Daniela Fiori
dc.creatorPavoni, Daniela Parada
dc.creatorKrieger, Marco Aurélio
dc.creatorMatthews, Keith
dc.creatorGoldenberg, Samuel
dc.creatorFragoso, Stenio Perdigão
dc.creatorDallagiovanna, Bruno
dc.date2019-12-12T19:46:38Z
dc.date2019-12-12T19:46:38Z
dc.date2012
dc.date.accessioned2023-09-26T21:32:33Z
dc.date.available2023-09-26T21:32:33Z
dc.identifierMÖRKING, Patrícia Alves et al. The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 107, n. 6, p. 790-799, 2012.
dc.identifier1678-8060
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37922
dc.identifier10.1590/s0074-02762012000600014
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872756
dc.descriptionTrypanosomes are parasitic protozoa in which gene expression is primarily controlled through the regulation of mRNA stability and translation. This post-transcriptional control is mediated by various families of RNA-binding proteins, including those with zinc finger CCCH motifs. CCCH zinc finger proteins have been shown to be essential to differentiation events in trypanosomatid parasites. Here, we functionally characterise TcZFP2 as a predicted post-transcriptional regulator of differentiation in Trypanosoma cruzi. This protein was detected in cell culture-derived amastigotes and trypomastigotes, but it was present in smaller amounts in metacyclic trypomastigote forms of T. cruzi. We use an optimised recombinant RNA immunopreciptation followed by microarray analysis assay to identify TcZFP2 target mRNAs. We further demonstrate that TcZFP2 binds an A-rich sequence in which the adenosine residue repeats are essential for high-affinity recognition. An analysis of the expression profiles of the genes encoding the TcZFP2-associated mRNAs throughout the parasite life cycle by microarray hybridisation showed that most of the associated mRNAs were upregulated in the metacyclic trypomastigote forms, also suggesting a role for TcZFP2 in metacyclic trypomastigote differentiation. Knockdown of the orthologous Trypanosoma brucei protein levels showed ZFP2 to be a positive regulator of specific target mRNA abundance.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectZinc Fingers
dc.subjectRNA-Binding Proteins
dc.subjectDedos de Zinc
dc.subjectProteínas de Unión al ARN
dc.subjectDedos de Zinco
dc.subjectProteínas de Ligação a RNA
dc.titleThe zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis
dc.typeArticle


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