High- and low-frequency transcutaneous electrical nerve stimulation delay sciatic nerve regeneration after crush lesion in the mouse

dc.creatorBaptista, Abrahão Fontes
dc.creatorGomes, Joyce R. S
dc.creatorOliveira, Juliana Teixeira
dc.creatorSantos, Soraia M. G.
dc.creatorSantos, Marcos André Vannier dos
dc.creatorMartinez, Ana Maria Blanco
dc.date2015-05-05T16:02:36Z
dc.date2015-05-05T16:02:36Z
dc.date2008
dc.date.accessioned2023-09-26T21:32:28Z
dc.date.available2023-09-26T21:32:28Z
dc.identifierBAPTISTA, A. F. et al. High- and low-frequency transcutaneous electrical nerve stimulation delay sciatic nerve regeneration after crush lesion in the mouse. Journal of the Peripheral Nervous System, v. 13, n. 1, p. 71-80, 2008.
dc.identifier1085-9489
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10239
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872751
dc.descriptionThe stimulation of peripheral nerve regeneration has been studied in different ways, including the use of electrical fields. The capacity of this modality to enhance nerve regeneration is influenced by the parameters used, including current type, frequency, intensity, and means of administration. Transcutaneous electrical nerve stimulation (TENS) is a frequently used form of administering electrical current to the body, but its effects on peripheral nerve regeneration are not known. This study assessed the influence of TENS on sciatic nerve regeneration, using a model of crush lesion in the mouse. Mice were stimulated 30 min a day, 5 days a week, for 5 weeks with both high- (100 Hz) and low- (4 Hz) frequency TENS. Control animals had the sciatic nerve crushed but were not stimulated. Assessment was performed weekly by functional analysis using the Static Sciatic Index for the mouse and at the end of the experiment by light and electron microscopy. The results showed that although there were no differences between the groups regarding the Static Sciatic Index values, TENS led to nerves with morphological signs of impaired regeneration. At light microscopy level, TENS nerves presented more axons with dark axoplasm, signs of edema, and a less organized cytoarchitecture. Electronmicrographs showed fewer and thinner thick myelinated fibers and increased number of Schwann cell nuclei. Myelinated axon diameters and density and diameter of nonmyelinated fibers were not affected by TENS, leading to the conclusion that this regimen of electrical stimulation leads to a delayed regeneration after a crush lesion of the sciatic nerve in the mouse. All these effects were more pronounced on high-frequency TENS nerves.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBlackwell Publishing
dc.rightsopen access
dc.subjectFunction recovery
dc.subjectHistology
dc.subjectMice
dc.subjectNerve regeneration
dc.subjectSciatic
dc.subjectTranscutaneous electric nerve stimulation
dc.subjectCompressão Nervosa
dc.subjectRegeneração Nervosa
dc.subjectNeuropatia Ciática
dc.subjectEstimulação Elétrica Nervosa Transcutânea
dc.subjectCamundongos
dc.titleHigh- and low-frequency transcutaneous electrical nerve stimulation delay sciatic nerve regeneration after crush lesion in the mouse
dc.typeArticle


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