Trypanosomatid Richness Among Rats, Opossums, and Dogs in the Caatinga Biome, Northeast Brazil, a Former Endemic Area of Chagas Disease

dc.creatorDario, Maria Augusta
dc.creatorFurtado, Carolina
dc.creatorLisboa, Cristiane Varella
dc.creatorOliveira, Felipe de
dc.creatorSantos, Filipe Martins
dc.creatorD’Andrea, Paulo Sérgio
dc.creatorRoque, André Luiz Rodrigues
dc.creatorXavier, Samanta Cristina das Chagas
dc.creatorJansen, Ana Maria
dc.date2022-07-21T19:01:08Z
dc.date2022-07-21T19:01:08Z
dc.date2022
dc.date.accessioned2023-09-26T21:29:21Z
dc.date.available2023-09-26T21:29:21Z
dc.identifierDARIO, Maria Augusta et al. Trypanosomatid Richness Among Rats, Opossums, and Dogs in the Caatinga Biome, Northeast Brazil, a Former Endemic Area of Chagas Disease. Frontiers in Cellular and Infection Microbiology, v. 12, Article 851903, p. 1 - 16, June 2022.
dc.identifier2235-2988
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/53911
dc.identifier10.3389/fcimb.2022.851903
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872572
dc.descriptionParasites are important components of the immense n-dimensional trophic network that connects all living beings because they, among others, forge biodiversity and deeply influence ecological evolution and host behavior. In this sense, the influence of Trypanosomatidae remains unknown. The aim of this study was to determine trypanosomatid infection and richness in rats, opossums, and dogs in the semiarid Caatinga biome. We submitted DNA samples from trypanosomatids obtained through axenic cultures of the blood of these mammals to mini exon multiplex-PCR, Sanger, and next-generation sequencing targeting the 18S rDNA gene. Phylogenetic analyses were performed to identify genetic diversity in the Trypanosomatidae family. Shannon, Simpson, equability, and beta-diversity indices were calculated per location and per mammalian host. Dogs were surveyed for trypanosomatid infection through hemocultures and serological assays. The examined mammal species of this area of the Caatinga biome exhibited an enormous trypanosomatid species/genotypes richness. Ten denoised Operational Taxonomic Units (ZOTUs), including three species (Trypanosoma cruzi, Trypanosoma rangeli and Crithidia mellificae) and one Trypanosoma sp. five genotypes/ lineages (T. cruzi DTU TcI, TcII, and TcIV; T. rangeli A and B) and four DTU TcI haplotypes (ZOTU1, ZOTU2, ZOTU5, and ZOTU10 merged), as well as 13 Amplicon Sequence Variants (ASVs), including five species (T. cruzi, T. rangeli, C. mellificae, Trypanosoma dionisii, and Trypanosoma lainsoni), five genotypes/lineages (same as the ZOTUs) and six DTU TcI haplotypes (ASV, ASV1, ASV2, ASV3, ASV5 and ASV13), were identified in single and mixed infections. We observed that trypanosomatids present a broad host spectrum given that species related to a single host are found in other mammals from different taxa. Concomitant infections between trypanosomatids and new host-parasite relationships have been reported, and this immense diversity in mammals raised questions, such as how this can influence the course of the infection in these animals and its transmissibility. Dogs demonstrated a high infection rate by T. cruzi as observed by positive serological results (92% in 2005 and 76% in 2007). The absence of positive parasitological tests confirmed their poor infectivity potential but their importance as sentinel hosts of T. cruzi transmission.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectRiqueza de Trypanosomatidae
dc.subjectInfecção por Trypanosoma cruzi
dc.subjectHaplótipo T. cruzi DTU TcI
dc.subjectMamíferos sinantrópicos
dc.subjectCanis familiaris
dc.subjectBioma caatinga
dc.subjectTrypanosomatidae richness
dc.subjectTrypanosoma cruzi infection
dc.subjectT. cruzi DTU TcI haplotype
dc.subjectSynantropic mammals
dc.subjectCanis familiaris
dc.subjectCaatinga biome
dc.titleTrypanosomatid Richness Among Rats, Opossums, and Dogs in the Caatinga Biome, Northeast Brazil, a Former Endemic Area of Chagas Disease
dc.typeArticle


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