A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics

dc.creatorLavich, T. R.
dc.creatorCordeiro, R. S. B.
dc.creatorSilva, P. M. R.
dc.creatorMartins, M. A.
dc.date2019-12-10T13:38:51Z
dc.date2019-12-10T13:38:51Z
dc.date2005
dc.date.accessioned2023-09-26T21:29:06Z
dc.date.available2023-09-26T21:29:06Z
dc.identifierLAVICH, T. R. et al. A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, p. 445-451, 2005.
dc.identifier0100-879X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37746
dc.identifier10.1590/s0100-879x2005000300016
dc.identifier1414-431X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872557
dc.descriptionIt is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51 degrees C and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 microg/paw) and mice (125-500 microg/paw) with carrageenan led to a rapid hyperalgesic response of the ipsilateral paw that reached a plateau from 15 to 360 min after challenge. Pretreatment with indomethacin (4 mg/kg, i.p.) inhibited the phenomenon at all the times analyzed. Similarly, plantar stimulation of rats and mice with prostaglandin E2 (0.5 and 1 microg/paw) also resulted in rapid hyperalgesia which was first detected 15 min post-challenge. Finally, we observed that the MHP test was more sensitive than the classical Hargreaves' test, being able to detect about 4- and 10-fold lower doses of prostaglandin E2 and carrageenan, respectively. In conclusion, the MHP test is a simple and sensitive method for detecting peripheral hyperalgesia and analgesia in rats and mice. This test represents a low-cost alternative for the study of inflammatory pain in freely moving animals.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.rightsopen access
dc.subjectHyperalgesia
dc.subjectTeste de Hargreaves
dc.subjectTeste modificado da placa quente
dc.subjectCarragenina
dc.subjectProstaglandina E2
dc.subjectIndometacina
dc.subjectHyperalgesia
dc.subjectHargreaves’ test
dc.subjectModified hot-plate test
dc.subjectCarrageenan
dc.subjectProstaglandin E2
dc.subjectIndomethacin
dc.titleA novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
dc.typeArticle


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