Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria

dc.creatorEugenio, Mateus
dc.creatorMüller, Nathalia
dc.creatorFrasés, Susana
dc.creatorAlmeida-Paes, Rodrigo
dc.creatorLima, Luís Maurício T. R.
dc.creatorLemgruber, Leandro
dc.creatorFarina, Marcos
dc.creatorSouza, Wanderley de
dc.creatorSant’Anna, Celso
dc.date2019-04-24T14:18:17Z
dc.date2019-04-24T14:18:17Z
dc.date2016
dc.date.accessioned2023-09-26T21:27:07Z
dc.date.available2023-09-26T21:27:07Z
dc.identifierEUGENIO, Mateus et al. Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria. RSC Advances, p. 1-38, 2016.
dc.identifier2046-2069
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32715
dc.identifier10.1039/C5RA22727E
dc.identifier2046-2069
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872444
dc.descriptionHere, we provided the first evidences of yeast strains assisted Ag/AgCl-NPs production in vitro. The formed nanoparticles were characterized by spectroscopic and electron microscopy approaches. UV-Vis supported the biosynthesis. TEM analysis evidenced that nanoparticles mainly presented circular shape and their diameter varied mostly in the range from 2 to 10 nm. XRD analysis showed a crystalline structure, with diffraction peaks corresponding to metallic silver and silver chloride nanoparticles, and when analyzed by high-resolution transmission electron microscopy (HRTEM), instead of being round, (111) (octahedral) and (200) (cubic-) symmetry facets appeared systematically in one side of the nanoparticles. Analysis of ultra-thin sections by TEM indicated that the domain of the synthesis of Ag/AgCl-NPs were mainly between cell wall and the plasma membrane. By using 3D reconstruction obtained from focused ion beam scanning electron microscopy (FIB/SEM) the spatial distribution of the domains of nanoparticles synthesis was mapped and nanoaggregates of Ag/AgCl-NPs up 35 nm in diameter were observed. Extracellular synthesis also occurred; in accordance with the fact that conditioned media from yeast isolates were as efficient at producing Ag/AgCl-NPs as live-cell cultures. Exposure of Gram-positive Staphylococcus aureus and Gram-negative Klebsiella pneumoniae cultures to Ag/AgCl-NPs led to a strong growth inhibition as shown by optical density measurements. The Ag/AgCl-NPs described here have characteristics compatible with a strong potential for use in the biotechnology industry, particularly for biomedical applications.
dc.formatapplication/pdf
dc.languageeng
dc.publisherRoyal Society of Chemistry
dc.rightsopen access
dc.subjectBiotechnology
dc.subjectYeasts
dc.subjectMetallic nanoparticles
dc.subjectBiomimetic synthesis
dc.subjectSilver/silver chloride nanoparticles
dc.titleYeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
dc.typePreprint


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