Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil

dc.creatorRodrigues, Ana Claudia Souza
dc.creatorSantos, Ivson Cassiano de Oliveira
dc.creatorCampos, Caroline Conci
dc.creatorRezende, Isadora Nascimento
dc.creatorFerreira, Yanara Miranda
dc.creatorChaves, Claudia Elisabeth Volpe
dc.creatorSouza, Cláudio Marcos Rocha de
dc.creatorCarvalho-Assef, Ana Paula D’Alincourt
dc.creatorChang, Marilene Rodrigues
dc.date2019-09-05T12:47:06Z
dc.date2019-09-05T12:47:06Z
dc.date2019
dc.date.accessioned2023-09-26T21:21:36Z
dc.date.available2023-09-26T21:21:36Z
dc.identifierRODRIGUES, Ana Claudia Souza et al. Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-6, 2019.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/35349
dc.identifier10.1590/0074-02760180555
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872131
dc.descriptionBACKGROUND Polymyxins are currently used as a “last-line” treatment for multidrug-resistant Gram-negative infections. OBJECTIVES To identify the major mechanisms of resistance to polymyxin and compare the genetic similarity between multidrug resistant Klebsiella pneumoniae strains recovered from inpatients of public hospitals in the Mid-West of Brazil. METHODS 97 carbapenems non-susceptible K. pneumoniae w ere s tudied. β -lactamases ( blaOXA-48, blaKPC, blaNDM, blaCTX-M, blaSHV, blaTEM, blaIMP, blaVIM) and mcr-1 to mcr-5 genes were investigated by polymerase chain reaction (PCR). Mutations in chromosomal genes (pmrA, pmrB, phoP, phoQ, and mgrB) were screened by PCR and DNA sequencing. Clonal relatedness was established by using pulsed-field gel electrophoresis and multilocus sequence typing. FINDINGS K. pneumoniae isolates harbored blaKPC (93.3%), blaSHV (86.6%), blaTEM (80.0%), blaCTX-M (60%) genes. Of 15 K. pneumoniae resistant to polymyxin B the authors identified deleterious mutations in pmrB gene, mainly in T157P. None K. pneumoniae presented mcr gene variants. Genetic polymorphism analyses revealed 12 different pulsotypes. MAIN CONCLUSIONS Deleterious mutations in pmrB gene is the main chromosomal target for induction of polymyxin resistance in carbapenem-resistant K. pneumoniae in public hospitals in the Mid-West of Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectColistin
dc.subjectPolymyxins
dc.subjectMultidrug resistance
dc.subjectColistina
dc.subjectPolimixinas
dc.subjectResistência a Múltiplos Medicamentos
dc.titleNon-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
dc.typeArticle


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