Effect of diethylcarbamazine on chronic hepatic inflammation induced by alcohol in C57BL/6 mice

dc.creatorRocha, Sura Wanessa Santos
dc.creatorSilva, Bruna Santos
dc.creatorGomes, Fabiana Oliveira dos Santos
dc.creatorSoares e Silva, Amanda Karolina
dc.creatorRaposo, Catarina
dc.creatorBarbosa, Karla Patrícia Sousa
dc.creatorTorres, Dilênia de Oliveira Cipriano
dc.creatorSantos, Ana Célia Oliveira dos
dc.creatorPeixoto, Christina Alves
dc.date2018-04-12T12:39:19Z
dc.date2018-04-12T12:39:19Z
dc.date2012
dc.date.accessioned2023-09-26T21:18:42Z
dc.date.available2023-09-26T21:18:42Z
dc.identifierSANTOS ROCHA, S. W. et al. Effect of diethylcarbamazine on chronic hepatic inflammation induced by alcohol in C57BL/6 mice. European Journal of Pharmacology, v. 689, n. 1–3, p. 194–203, ago. 2012.
dc.identifier1879-0712
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25792
dc.identifier10.1016/j.ejphar.2012.05.044
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8871968
dc.descriptionEste estudo foi financiado pela Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), Centro de Pesquisas Aggeu Magalhães da Fundação Oswaldo Cruz em Recife, Brasil (CPqAM / FIOCRUZ), Centro de Tecnologia Estratégica no Nordeste (CETENE) e Instituto Nacional de Biologia Estrutural e Bioimagem (INBEB).
dc.descriptionSome pharmacological studies showed that diethylcarbamazine (DEC) interferes with the arachidonic acid metabolism, acting as an anti-inflammatory drug. The chronic alcohol consumption activates the hepatic inflammatory response associated to T-cell activation and overproduction of pro-inflammatory cytokines. The present work analyzed the anti-inflammatory effect of DEC on hepatic cells of alcoholic mice. Thirty-two male C57BL/6 mice were equally divided in the following groups: (a) control group (C), which received only water, (b) DEC-treated group, which received 50 mg/kg for 12 day (DEC50), (c) the alcoholic group (EtOH), submitted to only alcohol and (d) the alcohol-DEC treated group (EtOH50), submitted to alcohol plus DEC treatment after the induction of chronic alcoholism for 5 weeks. Biochemical analyses were performed and liver fragments were processed for light microscopy, transmission electron microscopy, immunohistochemical and western blot. The level of AST increased significantly in alcoholic group whereas a significant reduction of serum AST was detected in the EtOH50 group. Histological and ultrastructural analysis of alcoholic group showed evident hepatocellular damage, which was strikingly reduced in the alcoholic DEC-treated group. Immunohistochemistry results revealed highly expression of inflammatory markers as MDA, NF-κB, TNF-α, IL-6, VCAM and ICAM by the hepatic cells of the EtOH group; however no immunoreactivity for any of these cytokines was detected after DEC treatment. Western blot analyses showed increased MCP-1 and iNOS expression in EtOH group, which was significantly inhibited by DEC treatment. According to the present results, DEC can be a potential drug for the treatment of chronic inflammation induced by chronic alcoholism.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectDietilcarbamazina
dc.subjectAlcoolismo
dc.subjectMarcadores Inflamatórios
dc.subjectLesão hepática
dc.subjectDiethylcarbamazine
dc.subjectAlcoholism
dc.subjectInflammatory markers
dc.subjectHepatic injury
dc.subjectAnimais
dc.subjectDietilcarbamazina / farmacologia
dc.subjectDietilcarbamazina / uso terapêutico
dc.subjectEtanol / administração & dosagem
dc.subjectEtanol / efeitos adversos
dc.subjectHepatite Alcoólica / quimioterapia
dc.subjectHepatite Alcoólica / metabolismo
dc.subjectHepatite Alcoólica / patologia
dc.subjectMediadores / antagonistas da inflamação e inibidores
dc.subjectMediadores da Inflamação / metabolismo
dc.subjectMasculino
dc.subjectRatos
dc.subjectRatos, endogâmicos C57BL
dc.subjectResultado do tratamento
dc.titleEffect of diethylcarbamazine on chronic hepatic inflammation induced by alcohol in C57BL/6 mice
dc.typeArticle


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