Comparison of virulence of different Sporothrix schenckii clinical isolates using experimental murine model

dc.creatorBrito, Marcelly M. S.
dc.creatorConceição-Silva, Fatima
dc.creatorMorgado, Fernanda N.
dc.creatorRailbolt, Priscila S.
dc.creatorSchubach, Armando
dc.creatorSchubach, Tania P.
dc.creatorSchaffer, Guido M. V.
dc.creatorBorba, Cintia M .
dc.date2019-06-13T13:32:54Z
dc.date2019-06-13T13:32:54Z
dc.date2006
dc.date.accessioned2023-09-26T21:16:33Z
dc.date.available2023-09-26T21:16:33Z
dc.identifierBRITO, Marcelly M. S. et al. Comparison of virulence of different Sporothrix schenckii clinical isolates using experimental murine model. Medical Mycology, v. 45, p. 721-729, Dec. 2007.
dc.identifier1369-3786
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33491
dc.identifier10.1080/13693780701625131
dc.identifier1460-2709
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8871853
dc.descriptionThe virulence of two strains of Sporothrix schenckii isolated from patients with lymphocutaneous or disseminated sporotrichosis were examined in BALB/c mice (Group 1 and 2, respectively). The mice were inoculated subcutaneously into the left hind footpad with 4106 S. schenckii yeast cells in order to evaluate (i) the development of cutaneous lesions, (ii) signs of inactivity, (iii) weight loss, (iv) survival rates, (v) number of viable yeast cells in the lungs and spleen, (vi) splenic index, (vii) extent of organ lesions, and (viii) immunological responses. Comparison of the two groups showed more severe disease in Group 2 mice that developed significant weight and hair loss associated with inactivity and left hind footpad lesions that extended close to the testicular area. The histopathology and large number of viable microorganisms isolated from the spleen confirmed the higher invasive ability of this strain. Moreover, a decrease of an in vitro specific lymphoproliferative response and IFN-gamma production were observed over time in Group 2 mice. As a result, at the end of the experiment, the S. schenckiiantigen (Ss-Ag) response was considered negative with a stimulation index (SI) 2. In contrast, Group 1 mice presented a positive response to Ss-Ag (SI 14.1). These results confirm the existence of different virulence profiles in S. schenckii strains. In addition, the use of subcutaneous inoculation as a suitable route for verification of the pathogenicity of this fungus in the murine model was confirmed.
dc.description2020-06-13
dc.formatapplication/pdf
dc.languageeng
dc.publisherOxford University Press
dc.rightsopen access
dc.subjectSporothrix schenckii
dc.subjectVirulence
dc.subjectHuman isolates
dc.subjectExperimental inoculation
dc.subjectImmune response
dc.subjectSporotrichosis
dc.titleComparison of virulence of different Sporothrix schenckii clinical isolates using experimental murine model
dc.typeArticle


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