Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays

dc.creatorPires, Marize Quinhones
dc.creatorMadeira, Maria de Fátima
dc.creatorBittencourt, Vânia Rita Elias Pinheiro
dc.creatorPacheco, Raquel da Silva
dc.date2015-05-04T16:21:44Z
dc.date2015-05-04T16:21:44Z
dc.date2014
dc.date.accessioned2023-09-26T21:15:57Z
dc.date.available2023-09-26T21:15:57Z
dc.identifierPIRES, Marize Quinhones et al. Cutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 2, p. 243-246, 2014.
dc.identifier0037-8682
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10156
dc.identifier10.1590/0037-8682-0007-2013
dc.identifier1678-9849
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8871744
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas de Apoio a Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Instituto Kinder do Brasil (IKB).
dc.descriptionFAPERJ, CNPq, IKB
dc.descriptionIntroduction: During a diagnostic evaluation of canine visceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. Methods: Specifi c polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. Results: PCR assays for Leishmania subgenus identifi cation followed by RFLP-PCR analysis in biopsies from cutaneous lesions and the spleen confi rmed the presence of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in those fragments. Conclusions: This report reinforces the importance of using serological and molecular techniques in the epidemiological surveillance of canine populations in endemic areas in which both diseases are known to co-exist. In such cases, a reassessment of the control measures is required.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedade Brasileira de Medicina Tropical
dc.rightsopen access
dc.subjectVisceral leishmaniasis
dc.subjectCutaneous leishmaniasis
dc.subjectLeishmania braziliensis
dc.subjectLeishmania infantum
dc.subjectPolymerase chain reaction
dc.subjectLeishmaniose visceral
dc.subjectLeishmaniose cutânea
dc.subjectLeishmania braziliensis
dc.subjectLeishmania infantum
dc.subjectReação em cadeia da polimerase
dc.titleCutaneous and visceral leishmaniasis co-infection in dogs from Rio de Janeiro, Brazil: evaluation by specific PCR and RFLP-PCR assays
dc.typeArticle


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