| dc.creator | Amorim, M. R. | |
| dc.creator | Vargas, F. R. | |
| dc.creator | Llerena Junior, Juan Clinton | |
| dc.creator | Pombo-de-Oliveira, M. S. | |
| dc.date | 2015-06-09T16:41:43Z | |
| dc.date | 2015-06-09T16:41:43Z | |
| dc.date | 2007 | |
| dc.date.accessioned | 2023-09-26T21:15:00Z | |
| dc.date.available | 2023-09-26T21:15:00Z | |
| dc.identifier | AMORIM, M. R. et al. DNA extraction from fixed cytogenetic cell suspensions. Genetics and Molecular Research, Ribeirao Preto, v. 6, n. 3, p. 500-503, 2007. | |
| dc.identifier | 1676-5680 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/10749 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8871492 | |
| dc.description | AbstRACt. We developed a procedure for DNA extraction from
small volumes of fixed cell suspensions previously prepared for con-ventional cytogenetic analysis. Good quality DNA was isolated with a
fast and simple protocol using DNAzol reagent. This provided suitable
DNA for various types of molecular analyses, including polymerase
chain reaction, restriction fragment length polymorphism, denaturing
high-performance liquid chromatography, and direct sequencing. This
technique provides sufficient material for such test, which are important
for diagnosis of neoplastic diseases in pediatric patients. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Fundação Norte-Rio-Grandense de Pesquisa e Cultura | |
| dc.rights | open access | |
| dc.subject | DNA Extraction | |
| dc.subject | DNAzol | |
| dc.subject | Cell Suspension | |
| dc.subject | Cytogenetic | |
| dc.subject | DNA | |
| dc.subject | Citogenética | |
| dc.title | DNA extraction from fixed cytogenetic cell suspensions | |
| dc.type | Article | |
