Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease

dc.creatorTorrentes-Carvalho, Amanda
dc.creatorSánchez-Arcila, Juan Camilo
dc.creatorAzamor, Tamiris
dc.creatorBarbosa, Luciana Santos
dc.creatorHottz, Eugênio Damaceno
dc.creatorGandini, Mariana
dc.creatorBozza, Fernando Augusto
dc.creatorCunha, Rivaldo Venâncio da
dc.creatorPinto, Luzia Maria de Oliveira
dc.creatorDamasco, Paulo Vieira
dc.creatorAzeredo, Elzinandes Leal de
dc.date2020-04-23T13:49:29Z
dc.date2020-04-23T13:49:29Z
dc.date2020
dc.date.accessioned2023-09-26T21:14:59Z
dc.date.available2023-09-26T21:14:59Z
dc.identifierTORRENTES-CARVALHO, Amanda et al. Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease. Scientific Reports, v. 10, n. 6351, p. 1-13, 14 Apr. 2020.
dc.identifier2045-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/40932
dc.identifier10.1038/s41598-020-62776-4
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8871489
dc.descriptionDengue virus (DENV) co-circulation in Brazil represents a challenge for treatment and vaccine development. Despite public health impact, the occurrence of coinfections with other viruses is a common event. Increased T cell activation and altered inflammatory response are found during DENV coinfection with Human Immunodeficiency Virus (HIV) impacting HIV-pathogenesis. Even with Antiretroviral therapy (ART), HIV- treated patients had chronic immune activation and lymphocyte apoptosis. However, apoptotic mechanisms have not been investigated during coinfection with DENV. Our attention was attracted to apoptotic cell markers expressions in PBMCs from DENV and DENV/HIV coinfected patients. We found CD4/CD8 ratio inversion in most coinfected patients. CD4 T and CD8 T-cell subsets from DENV and DENV/HIV groups expressed low levels of anti-apoptotic protein Bcl-2. Furthermore, CD8 CD95 double positive cells frequency expressing low levels of Bcl-2 were significantly higher in these patients. Additionally, the density of Bcl-2 on classical monocytes (CD14++CD16-) was significantly lower during DENV infection. Upregulation of pro-apoptotic proteins and anti-apoptotic proteins were found in DENV and DENV/HIV, while catalase, an antioxidant protein, was upregulated mainly in DENV/HIV coinfection. These findings provide evidence of apoptosis triggering during DENV/HIV coinfection, which may contribute to knowledge of immunological response during DENV acute infection in HIV-patients treated with ART.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNature Research
dc.rightsopen access
dc.subjectApoptose
dc.subjectLeucócitos de sangue mononuclear
dc.subjectPacientes com HIV
dc.subjectDengue aguda
dc.subjectApoptosis
dc.subjectMononuclear blood leukocytes
dc.subjectHIV patients
dc.subjectDengue acute disease
dc.titleApoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
dc.typeArticle


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