Validation of a direct agglutination test prototype kit for the diagnosis of visceral leishmaniasis

dc.creatorOliveira, Edward José de
dc.creatorSaliba, Soraya Wilke
dc.creatorSaliba, Juliana Wilke
dc.creatorRabello, Ana Lucia Teles
dc.date2018-11-26T17:09:30Z
dc.date2018-11-26T17:09:30Z
dc.date2013
dc.date.accessioned2023-09-26T21:14:54Z
dc.date.available2023-09-26T21:14:54Z
dc.identifierOLIVEIRA, Edward José de et al. Validation of a direct agglutination test prototype kit for the diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg., v. 107, n. 4, p. 243-247, 2013
dc.identifier0035-9203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30200
dc.identifier10.1093/trstmh/trt004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8871471
dc.descriptionBackground: A freeze-dried antigen was developed with Leishmania (L.) infantum and used for the production of a prototype direct agglutination test kit for the laboratory diagnosis of visceral leishmaniasis (VL), called DAT-LPC. On this study the diagnosis validity of this prototype was performed. Methods: To evaluate the sensitivity and specificity 103 samples from Brazilian patients with VL and 110 samples from patients with other parasitic infections, and healthy subjects were assayed with DAT-LPC and DAT-KIT (Royal Tropical Institute, Amsterdam, NL). Additionally, the results of 103 samples of VL patients based on two agglutination tests were transformed in Log10 and correlated. Results: The DAT-LPC showed a sensitivity of 99.0%, specificity of 98.2% and diagnosis validity of 98.6%, which were similar to those found by the DAT-KIT (p.0.05). Moreover, there was positive correlation between the positive titers obtained by DAT-LPC and by DAT-KIT (Spearman correlation coefficient of 0.75 p ¼ 0.0001). Conclusions: DAT-LPC showed thermal stability and diagnosis performance similar to those of the DAT-KIT. Our results suggest that DAT-LPC is a robust, simple, equipment-independent and efficient tool for the diagnosis of VL and should thus replace the IFAT as routine diagnostic test in the Brazilian public health system.
dc.description2100-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherRoyal Society of Tropical Medicine and Hygiene
dc.rightsrestricted access
dc.subjectLeishmaniose visceral
dc.subjectdiagnostico sorologico
dc.subjectKit de protótipo
dc.subjectDAT-LPC
dc.subjectVisceral leishmaniasis
dc.subjectSerological diagnosis
dc.subjectPrototype kit
dc.subjectDAT-LPC
dc.titleValidation of a direct agglutination test prototype kit for the diagnosis of visceral leishmaniasis
dc.typeArticle


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