Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils

dc.creatorPrates, Deboraci Brito
dc.creatorSantos, Théo de Araújo
dc.creatorLuz, Nívea Farias
dc.creatorAndrade, Bruno de Bezerril
dc.creatorCosta, Jaqueline França
dc.creatorAfonso, Lilian Maria
dc.creatorClarêncio, Jorge
dc.creatorMiranda, José Carlos
dc.creatorBozza, Patrícia Torres
dc.creatorReis, George A. dos
dc.creatorBrodskyn, Claudia Ida
dc.creatorBarral Netto, Manoel
dc.creatorBorges, Valeria de Matos
dc.creatorBarral, Aldina Maria Prado
dc.date2014-02-17T19:36:00Z
dc.date2014-02-17T19:36:00Z
dc.date2011
dc.date.accessioned2023-09-26T21:14:37Z
dc.date.available2023-09-26T21:14:37Z
dc.identifierPRATES, D. B. et al. Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils. Journal of Leukocyte Biology, v. 90, n. 3, p.575-582, 2011.
dc.identifier1938-3673
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7316
dc.identifier10.1189/jlb.0211105
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8871390
dc.descriptionNeutrophils are considered the host's first line of defense against infections and have been implicated in the immunopathogenesis of Leishmaniasis. Leishmania parasites are inoculated alongside vectors' saliva, which is a rich source of pharmacologically active substances that interfere with host immune response. In the present study, we tested the hypothesis that salivary components from Lutzomyia longipalpis, an important vector of visceral Leishmaniasis, enhance neutrophil apoptosis. Murine inflammatory peritoneal neutrophils cultured in the presence of SGS presented increased surface expression of FasL and underwent caspase-dependent and FasL-mediated apoptosis. This proapoptosis effect of SGS on neutrophils was abrogated by pretreatment with protease as well as preincubation with antisaliva antibodies. Furthermore, in the presence of Leishmania chagasi, SGS also increased apoptosis on neutrophils and increased PGE(2) release and decreased ROS production by neutrophils, while enhancing parasite viability inside these cells. The increased parasite burden was abrogated by treatment with z-VAD, a pan caspase inhibitor, and NS-398, a COX-2 inhibitor. In the presence of SGS, Leishmania-infected neutrophils produced higher levels of MCP-1 and attracted a high number of macrophages by chemotaxis in vitro assays. Both of these events were abrogated by pretreatment of neutrophils with bindarit, an inhibitor of CCL2/MCP-1 expression. Taken together, our data support the hypothesis that vector salivary proteins trigger caspase-dependent and FasL-mediated apoptosis, thereby favoring Leishmania survival inside neutrophils, which may represent an important mechanism for the establishment of Leishmania infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociety for Leukocyte Biology
dc.rightsopen access
dc.subjectApoptose
dc.subjectLeishmaniose/imunologia
dc.subjectNeutrófilos/patologia
dc.subjectNeutrófilos/parasitologia
dc.subjectPsychodidae/imunologia
dc.subjectSaliva/imunologia
dc.subjectAnimais
dc.subjectCaspases/metabolismo
dc.subjectQuimiotaxia
dc.subjectQuimiocina CCL2/metabolismo
dc.subjectFeminino
dc.subjectProteína Ligante Fas/metabolismo
dc.subjectInterações Hospedeiro-Parasita
dc.subjectImmunoblotting
dc.subjectLeishmania
dc.subjectLeishmaniose/parasitologia
dc.subjectMacrófagos/imunologia
dc.subjectMasculino
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos C57BL
dc.subjectNeutrófilos/imunologia
dc.subjectPsychodidae/parasitologia
dc.subjectEspécies de Oxigênio Reativas/metabolismo
dc.subjectSaliva/química
dc.subjectSaliva/parasitologia
dc.subjectGlândulas Salivares/citologia
dc.subjectGlândulas Salivares/imunologia
dc.subjectGlândulas Salivares/parasitologia
dc.titleLutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils
dc.typeArticle


Este ítem pertenece a la siguiente institución