Selective and Sensitive Pull Down of Amyloid Fibrils Produced in Vitro and in Vivo by the Use of Pentameric-Thiophene-Coupled Resins

dc.creatorWreden, Anna Beatriz
dc.creatorFernandes, Luiza
dc.creatorKelley, Mirian
dc.creatorPereira-Neves, Antonio
dc.creatorMoreira, Caroline S.
dc.creatorRocha, David R. da
dc.creatorPalhano, Fernando L.
dc.date2019-11-13T15:06:25Z
dc.date2019-11-13T15:06:25Z
dc.date2018
dc.date.accessioned2023-09-26T21:14:04Z
dc.date.available2023-09-26T21:14:04Z
dc.identifierWREDEN, Anna Beatriz et al. Selective and Sensitive Pull Down of Amyloid Fibrils Produced in Vitro and in Vivo by the Use of Pentameric-Thiophene-Coupled Resins. ACS Chemical Neuroscience, v. 9, n. 11, p. 2807-2814, 2018.
dc.identifier1948-7193
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37033
dc.identifier10.1021/acschemneuro.8b00222
dc.identifier1948-7193
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8871245
dc.descriptionProtein aggregation is a hallmark of several degenerative diseases, including Alzheimer's disease, Parkinson's disease and familial amyloidosis (Finnish type) (FAF). A method to isolate and detect amyloids is desired for the diagnosis of amyloid diseases. Here, we report the synthesis of pentameric thiophene amyloid ligand (p-FTAA) linked to agarose resin for selective purification of amyloid aggregates produced in vitro and in vivo. Using amyloid fibrils produced in vitro from α-synuclein, gelsolin, and Aβ1-40 and gelsolin amyloid aggregates extracted from tissue homogenates of a mouse model of FAF, we observed that p-FTAA resin was able to pull down amyloid aggregates. The functionalized resin was also able to pull down oligomers produced in vitro from the A30P variant of α-synuclein. The methodology described here can be useful for the diagnosis of amyloidogenic disease and also can be used to purify amyloid fibrils from biological samples, rendering the fibrils available for more accurate structural and biochemical characterization.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectWestern blot
dc.subjectAlpha-synuclein
dc.subjectAmyloid
dc.subjectAmyloid-beta
dc.subjectGelsolin
dc.subjectPentameric thiophene
dc.subjectProtein aggregation
dc.subjectWestern Blottin
dc.subjectAlfa-Sinucleína
dc.subjectAmiloide
dc.subjectGelsolina
dc.subjectAgregação Patológica de Proteínas
dc.titleSelective and Sensitive Pull Down of Amyloid Fibrils Produced in Vitro and in Vivo by the Use of Pentameric-Thiophene-Coupled Resins
dc.typeArticle


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