Kinetics of mesenchymal and hematopoietic stem cells mobilization by G-CSF and its impact on the cytokine microenvironment in primary cultures

dc.creatorGarcia, Nadja Pinto
dc.creatorLeon, Elisa Brosina de
dc.creatorCosta, Allyson Guimarães da
dc.creatorTarragô, Andréa Monteiro
dc.creatorPimentel, João Paulo
dc.creatorFraporti, Liziara
dc.creatorAraujo, Fernanda Fortes de
dc.creatorCampos, Fernanda Magalhães Freire
dc.creatorCarvalho, Andréa Teixeira
dc.creatorMartins Filho, Olindo Assis
dc.creatorMalheiro, Adriana
dc.date2015-05-25T12:59:44Z
dc.date2015-05-25T12:59:44Z
dc.date2014
dc.date.accessioned2023-09-26T21:12:34Z
dc.date.available2023-09-26T21:12:34Z
dc.identifierGARCIA, Nadja Pinto et al. Kinetics of mesenchymal and hematopoietic stem cells mobilization by G-CSF and its impact on the cytokine microenvironment in primary cultures. Cellular Immunology, vol. 293, n. 2015, p.1–9, 2014
dc.identifier0008-8749
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10486
dc.identifier10.1016/j.cellimm.2014.09.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8870860
dc.descriptionIn this study, we demonstrate that G-CSF administration triggers distinct kinetics of stem cell-SC mobilization with early raise of hematopoietic-HSC and late increase of mesenchymal-MSC in bone marrow-BM and peripheral blood-PB. The cytokine microenvironment observed following primary cultures showed an overall G-CSF dose-dependent profile with a clear mixed pro-inflammatory/regulatory pattern. Moreover, primary cultures performed at the peak of MSC/HSC ratio, showed distinct cytokine patterns, with higher IL-10, TNF-a and IL-17A observed for BM and enhanced IL-10, IL-2 and IFN-c for PB harvested cells. Positive correlation was observed between BM-MSC and the levels of TNF-a, IL-10 and IL-17A whereas negative correlation was found between IL-10 and BM-HSC. An opposite association was observed between IL-10 and PB-HSC. Our results support the hypothesis that MSC and HSC harvested from BM and PB display differential functional properties that should be considered when electing the SC sources available for cell therapy applied in clinical protocols.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Inc.
dc.rightsrestricted access
dc.subjectMesenchymal
dc.subjectHematopoietic Stem
dc.subjectcells mobilization
dc.subjectCytokines
dc.titleKinetics of mesenchymal and hematopoietic stem cells mobilization by G-CSF and its impact on the cytokine microenvironment in primary cultures
dc.typeArticle


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