Experimental infection of dogs with Leishmania and saliva as a model to study Canine Visceral Leishmaniasis

dc.creatorCosta, Dirceu Joaquim
dc.creatorCarvalho, Rayssa Maria de Araujo
dc.creatorAbbehusen, Melissa Moura Costa
dc.creatorTeixeira, Clarissa Romero
dc.creatorPitombo, Maiana A
dc.creatorTrigo, Joelma
dc.creatorNascimento, Flávia Raquel Fernandes do
dc.creatorAmorim, Lucilene
dc.creatorSilva, Ana Lucia Abreu
dc.creatorCruz, Maria do Socorro Pires
dc.creatorMiranda, José Carlos
dc.creatorFukutani, Kiyoshi Ferreira
dc.creatorOliveira, Camila Indiani de
dc.creatorBarral, Aldina Maria Prado
dc.creatorBarral Netto, Manoel
dc.creatorBrodskyn, Claudia Ida
dc.date2014-02-18T19:23:15Z
dc.date2014-02-18T19:23:15Z
dc.date2013
dc.date.accessioned2023-09-26T21:12:27Z
dc.date.available2023-09-26T21:12:27Z
dc.identifierCOSTA, D. J. et al. Experimental infection of dogs with Leishmania and saliva as a model to study Canine Visceral Leishmaniasis. Plos One, v. 8, p. e60535, 2013.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7330
dc.identifier10.1371/journal.pone.0060535
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8870832
dc.descriptionBACKGROUND: Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs is very important to test vaccine candidates, mainly regarding those that use salivary proteins from the vector and new therapeutical approaches. METHODOLOGY/PRINCIPAL FINDINGS: In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 1×10(7) parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,35×10(5) parasites in their lymph nodes. An increase of IFN-γ and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection. CONCLUSION: The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstitut Armand Frappier
dc.rightsopen access
dc.subjectDoenças do Cão/parasitologia
dc.subjectLeishmania infantum/fisiologia
dc.subjectLeishmaniose Visceral/veterinária
dc.subjectSaliva/parasitologia
dc.subjectAnimais
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectCitocinas/sangue
dc.subjectModelos Animais de Doenças
dc.subjectDoenças do Cão/sangue
dc.subjectDoenças do Cão/transmissão
dc.subjectCães
dc.subjectFeminino
dc.subjectImunoglobulina G/sangue
dc.subjectLeishmania infantum/imunologia
dc.subjectLeishmaniose Visceral/sangue
dc.subjectLeishmaniose Visceral/parasitologia
dc.subjectLeishmaniose Visceral/transmissão
dc.subjectCarga Parasitária
dc.subjectPsychodidae/parasitologia
dc.subjectGlândulas Salivares/parasitologia
dc.titleExperimental infection of dogs with Leishmania and saliva as a model to study Canine Visceral Leishmaniasis
dc.typeArticle


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