Production of MMP-9 and inflammatory cytokines by Trypanosoma cruzi-infected macrophages

dc.creatorPinho, Rosa Teixeira de
dc.creatorSilva, Wellington Seguins da
dc.creatorCôrtes, Luzia Monteiro de Castro
dc.creatorSouza, Periela da Silva Vasconcelos
dc.creatorSoares, Renata Oliveira de Araujo
dc.creatorAlves, Carlos Roberto
dc.date2015-05-04T16:34:36Z
dc.date2015-05-04T16:34:36Z
dc.date2014
dc.date.accessioned2023-09-26T21:12:05Z
dc.date.available2023-09-26T21:12:05Z
dc.identifierPINHO, Rosa Teixeira de et al. Production of MMP-9 and inflammatory cytokines by Trypanosoma cruzi-infected macrophages. Experimental Parasitology, v.147, p.72–80, 2014.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10182
dc.identifier10.1016/j.exppara.2014.09.003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8870738
dc.descriptionMatrix metalloproteinases (MMPs) constitute a large family of Zn2+ and Ca2+ dependent endopeptidases implicated in tissue remodeling and chronic inflammation. MMPs also play key roles in the activation of growth factors, chemokines and cytokines produced by many cell types, including lymphocytes, granulocytes, and, in particular, activated macrophages. Their synthesis and secretion appear to be important in a number of physiological processes, including the inflammatory process. Here, we investigated the interaction between human and mouse macrophages with T. cruzi Colombian and Y strains to characterize MMP-9 and cytokine production in this system. Supernatants and total extract of T. cruzi infected human and mouse macrophages were obtained and used to assess MMP-9 profile and inflammatory cytokines. The presence of metalloproteinase activity was determined by zymography, enzyme-linked immunosorbent assay and immunoblotting assays. The effect of cytokines on MMP-9 production in human macrophages was verified by previous incubation of cytokines on these cells in culture, and analyzed by zymography. We detected an increase in MMP-9 production in the culture supernatants of T. cruzi infected human and mouse macrophages. The addition of IL-1β or TNF-α to human macrophage cultures increased MMP-9 production. In contrast, MMP-9 production was down-modulated when human macrophage cultures were treated with IFN-γ or IL-4 before infection. Human macrophages infected with T. cruzi Y or Colombian strains produced increased levels of MMP-9, which was related to the production of cytokines such as IL-1β, TNF-α and IL-6.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectCitocinas
dc.subjectTrypanosoma cruzi
dc.subjectMatrix metalloproteinase-9
dc.subjectY strain
dc.subjectColombian strain
dc.subjectHuman macrophages
dc.subjectMouse macrophages
dc.subjectTHP-1 macrophages
dc.subjectCytokines
dc.subjectTrypanosoma cruzi
dc.subjectCitocinas
dc.subjectCiências Biológicas
dc.subjectCitocinas
dc.titleProduction of MMP-9 and inflammatory cytokines by Trypanosoma cruzi-infected macrophages
dc.typeArticle


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