Metacyclogenesis modulates the ability of Leishmania promastigotes to induce IL-12 production in human mononuclear cells

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSartori, Alexandrina
dc.creatorOliveira, Milton A. P.
dc.creatorScott, Phillip
dc.creatorTrinchieri, Giorgio
dc.date2014-05-27T11:18:19Z
dc.date2016-10-25T18:14:51Z
dc.date2014-05-27T11:18:19Z
dc.date2016-10-25T18:14:51Z
dc.date1997-12-01
dc.date.accessioned2017-04-06T00:51:07Z
dc.date.available2017-04-06T00:51:07Z
dc.identifierJournal of Immunology, v. 159, n. 6, p. 2849-2857, 1997.
dc.identifier0022-1767
dc.identifierhttp://hdl.handle.net/11449/65320
dc.identifierhttp://acervodigital.unesp.br/handle/11449/65320
dc.identifier2-s2.0-0031571866
dc.identifierhttp://www.jimmunol.org/content/159/6/2849.long
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/887055
dc.descriptionImmunity against the intracellular protozoan Leishmania species is highly dependent on Th1 development. We have previously shown that IL-12 is a powerful and probably obligatory factor for Th1 cell generation and proliferation. We also observed that the experimental infection of C3H and BALB/c mice with Leishmania major is associated with IL-12 production in vivo. Now we demonstrate that metacyclic L. major promastigotes are poor inducers of IL-12 in vitro in fresh human PBMC and monocytes. In addition, we show that the ability of this pathogen to induce IL-12 and other cytokines is modulated by the metacyclogenic process, which had previously not been recognized. In contrast to the infective parasites (metacyclic promastigotes), the procyclic promastigotes collected at the logarithmic phase of the culture displayed a striking ability to induce IL-12, IFN-γ, TNF-α, and IL-10. Despite this differential effect of procyclic and metacyclic parasites in terms of IL-12 induction, both stages were inhibitory for IL-12 production induced by Staphylococcus aureus. The ability of procyclic promastigotes and, to a much lesser extent, that of metacyclic promastigotes to induce IL-12 were enhanced by pretreatment of monocytes in a cytokine milieu containing IFN-γ, IL-4, IL-13, or granulocyte-macrophage CSF or. by neutralization of endogenous IL-10. Our results suggest the development of an evasion mechanism as the Leishmania promastigotes mature to infectious forms and the possi-bility of using Ags derived from procyclic promastigotes for immunization procedures. Copyright © 1997 by The American Association of Immunologists.
dc.languageeng
dc.relationJournal of Immunology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectgamma interferon
dc.subjectinterleukin 10
dc.subjectinterleukin 12
dc.subjecttumor necrosis factor alpha
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectgrowth, development and aging
dc.subjecthuman
dc.subjectimmunology
dc.subjectLeishmania major
dc.subjectmonocyte
dc.subjectmouse
dc.subjectparasitology
dc.subjectAnimals
dc.subjectHumans
dc.subjectInterferon Type II
dc.subjectInterleukin-10
dc.subjectInterleukin-12
dc.subjectMice
dc.subjectMonocytes
dc.subjectTumor Necrosis Factor-alpha
dc.titleMetacyclogenesis modulates the ability of Leishmania promastigotes to induce IL-12 production in human mononuclear cells
dc.typeOtro


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