Oxalate determination in urine using an immobilized enzyme on Sorghum vulgare seeds in a flow injection conductimetric system

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOliveira Neto, Graciliano de
dc.creatorTubino, Matthieu
dc.creatorGodinho, Oswaldo Espirito Santo
dc.creatorKubota, Lauro Tatsuo
dc.creatorFernandes, João Roberto
dc.date2014-05-27T11:18:18Z
dc.date2016-10-25T18:14:47Z
dc.date2014-05-27T11:18:18Z
dc.date2016-10-25T18:14:47Z
dc.date1997-12-01
dc.date.accessioned2017-04-06T00:50:53Z
dc.date.available2017-04-06T00:50:53Z
dc.identifierJournal of the Brazilian Chemical Society, v. 8, n. 1, p. 47-51, 1997.
dc.identifier0103-5053
dc.identifierhttp://hdl.handle.net/11449/65291
dc.identifierhttp://acervodigital.unesp.br/handle/11449/65291
dc.identifier10.1590/S0103-50531997000100009
dc.identifierS0103-50531997000100009
dc.identifierWOS:A1997WP60200009
dc.identifier2-s2.0-3042869073.pdf
dc.identifier2-s2.0-3042869073
dc.identifierhttp://dx.doi.org/10.1590/S0103-50531997000100009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/887026
dc.descriptionA flow-injection (FI) method was developed for the determination of oxalate in urine. It was based on the use of oxalate oxidase (E.C. 1.2.3.4) immobilized on ground seeds of the BR-303 Sorghum vulgare variety. A reactor was filled with this activated material, and the samples (200 μL) containing oxalate were passed through it, carried by a deionized water flow. The carbon dioxide produced by the enzyme reaction permeated through a microporous PTFE membrane, and was received in a water acceptor stream, promoting conductivity changes proportional to the oxalate concentration in the sample. The results obtained showed a useful linear range from 0.05 to 0.50 mmol dm-3. The proposed method, when compared with the Sigma enzymatic procedure, showed good correlation (Y = 0.006(±0.016) + 0.98(±0.019)X; r = 0.9995, Y = conductivity in μS, and X = concentration in mmol dm-3), selectivity, and sensitivity. The new immobilization approach promotes greater stability, allowing oxalate determination for 6 months. About 13 determinations can be performed per hour. The precision of the proposed method is about ± 3.2 % (r.s.d).
dc.languageeng
dc.relationJournal of the Brazilian Chemical Society
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectConductimetry
dc.subjectEnzyme immobilization
dc.subjectFlow injection
dc.subjectOxalate determination
dc.subjectOxalate oxidase
dc.subjectSorghum vulgare
dc.titleOxalate determination in urine using an immobilized enzyme on Sorghum vulgare seeds in a flow injection conductimetric system
dc.typeOtro


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