Molecular epidemiology of HIV-1 in Brazil: high prevalence of HIV-1 subtype B and identification of an HIV-1 subtype D infection in the city of Rio de Janeiro, Brazil

dc.creatorMorgado, Mariza Gonçalves
dc.creatorGuimarães, Monick Lindenmeyer
dc.creatorGripp, Carmen B. G.
dc.creatorCosta, Catia Ines
dc.creatorNeves Junior, Ivan
dc.creatorVeloso, Valdiléa G.
dc.creatorCarvalho, Maria Inês Linhares de
dc.creatorCastello-Branco, Luis Roberto Ribeiro
dc.creatorBastos, Francisco Inácio Pinkusfeld Monteiro
dc.creatorKuiken, Carla
dc.creatorCastilho, Euclides Ayres de
dc.creatorCastro Filho, Bernardo Galvão
dc.creatorBongertz, Vera
dc.date2014-08-12T17:52:20Z
dc.date2014-08-12T17:52:20Z
dc.date1998
dc.date.accessioned2023-09-26T21:09:52Z
dc.date.available2023-09-26T21:09:52Z
dc.identifierMORGADO, Mariza Gonçalves et al. Molecular epidemiology of HIV-1 in Brazil: high prevalence of HIV-1 subtype B and identification of an HIV-1 subtype D infection in the city of Rio de Janeiro, Brazil. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, v. 18, n. 5, p. 488-494, 1998.
dc.identifier1077-9450
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8192
dc.identifier10.1097/00042560-199808150-00011
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8870167
dc.descriptionHIV-1-positive individuals were recruited from January 1993 to December 1996 from several cohorts receiving follow-up in the city of Rio de Janeiro, Brazil, to evaluate HIV-1 genetic variability and the potential association with modes of transmission. HIV-1 subtyping was carried out using the heteroduplex mobility assay (HMA), and those samples corresponding to the typical Brazilian subtype B variant were further identified based on the Fok I restriction fragment length polymorphism (RFLP). DNA sequencing was performed to evaluate one case of subtype D infection. From the 131 HIV-1-positive individuals analyzed, 106 (80.9%) could be identified as infected by subtype B and 20 (15.3%) by subtype F. One of the samples (0.8%) was classified as subtype D. DNA samples from 4 patients (3.0%) did not yield polymerase chain reaction (PCR)-amplified products to be typed. Based on the Fok I RFLP, 39 of the 106 subtype B samples (37%) were identified as corresponding to the typical Brazilian subtype B variant containing the GWGR motif at the tip of the V3 loop. No statistically significant association could be detected between HIV-I subtypes and modes of transmission, exposure categories, or gender. This is the first reported case of HIV-1 subtype D infection in Brazil
dc.formatapplication/pdf
dc.languageeng
dc.publisherLippincott-Raven Publishers, Philadelphia
dc.rightsopen access
dc.subjectHIV-1 subtypes in Brazil
dc.subjectHeteroduplex mobility assay
dc.subjectHIV-1 subtype D infection
dc.subjectInfecções por HIV/epidemiologia
dc.subjectHIV-1/classificação
dc.subjectSequência de Aminoácidos
dc.subjectBrasil/epidemiologia
dc.subjectEstudos de Coortes
dc.subjectSequência Consenso
dc.subjectDNA Viral/química
dc.subjectFeminino
dc.subjectProdutos do Gene env/química
dc.subjectVariação Genética
dc.subjectInfecções por HIV/transmissão
dc.subjectHIV-1/genética
dc.subjectHumanos
dc.subjectMasculino
dc.subjectEpidemiologia Molecular
dc.subjectDados de Sequência Molecular
dc.subjectÁcidos Nucleicos Heteroduplexes
dc.subjectFilogenia
dc.subjectReação em Cadeia da Polimerase
dc.subjectPolimorfismo de Fragmento de Restrição
dc.subjectPrevalência
dc.subjectAlinhamento de Sequência
dc.subjectDistribuição por Sexo
dc.subjectPopulação Urbana
dc.titleMolecular epidemiology of HIV-1 in Brazil: high prevalence of HIV-1 subtype B and identification of an HIV-1 subtype D infection in the city of Rio de Janeiro, Brazil
dc.typeArticle


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