GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide

dc.creatorLima, D. C.
dc.creatorDuarte, Fábio T.
dc.creatorMedeiros, Viviane Katielly Silva
dc.creatorCarvalho, Paulo Costa
dc.creatorNogueira, Fábio Cesar Sousa
dc.creatorAraujo, Gabriel Duarte Tavares
dc.creatorDumont, Gilberto Barbosa
dc.creatorMedeiros, Sivia Regina Batistuzzo de
dc.date2017-07-28T16:16:46Z
dc.date2017-07-28T16:16:46Z
dc.date2016
dc.date.accessioned2023-09-26T21:08:46Z
dc.date.available2023-09-26T21:08:46Z
dc.identifierLIMA, D. C. et al. GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide. Scientific Reports, v. 6, n. 28174, p. 1-12, Jun. 16.
dc.identifier2045-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20446
dc.identifier10.1038/srep28174
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869875
dc.descriptionChromobacterium violaceum is a free-living bacillus with several genes that enables it survival under different harsh environments such as oxidative and temperature stresses. Here we performed a labelfree quantitative proteomic study to unravel the molecular mechanisms that enable C. violaceum to survive oxidative stress. To achieve this, total proteins extracted from control and C. violaceum cultures exposed during two hours with 8mM hydrogen peroxide were analyzed using GeLC-MS proteomics. Analysis revealed that under the stress condition, the bacterium expressed proteins that protected it from the damage caused by reactive oxygen condition and decreasing the abundance of proteins responsible for bacterial growth and catabolism. GeLC-MS proteomics analysis provided an overview of the metabolic pathways involved in the response of C. violaceum to oxidative stress ultimately aggregating knowledge of the response of this organism to environmental stress. This study identified approximately 1500 proteins, generating the largest proteomic coverage of C. violaceum so far. We also detected proteins with unknown function that we hypothesize to be part of new mechanisms related to oxidative stress defense. Finally, we identified the mechanism of clustered regularly interspaced short palindromic repeats (CRISPR), which has not yet been reported for this organism.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNature Publishing Group
dc.rightsopen access
dc.subjectProteômica
dc.subjectChromobacterium
dc.subjectProteomics
dc.subjectClustered Regularly Interspaced Short Palindromic Repeats
dc.subjectHydrogen Peroxide
dc.subjectChromobacterium
dc.subjectProteómica
dc.subjectRepeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
dc.subjectPeróxido de Hidrógeno
dc.subjectChromobacterium
dc.subjectRepetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
dc.subjectPeróxido de Hidrogênio
dc.titleGeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide
dc.typeArticle


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