Outbreak of acute Chagas disease occurred in the state of Bahia, Brazil

dc.creatorTsunetsugu-Yokota, Yasuko
dc.creatorMorikawa, Yuko
dc.creatorIsogai, Maya
dc.creatorKawana-Tachikawa, Ai
dc.creatorOdawara, Takashi
dc.creatorNakamura, Tetsuya
dc.creatorGrassi, Maria Fernanda Rios
dc.creatorAutran, Brigitte
dc.creatorIwamoto, Aikichi
dc.date2014-10-07T18:03:29Z
dc.date2014-10-07T18:03:29Z
dc.date2003
dc.date.accessioned2023-09-26T21:08:44Z
dc.date.available2023-09-26T21:08:44Z
dc.identifierTSUNETSUGU-YOKOTA, Y. et al. Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs. Journal of Virology, v. 77, n. 19, p. 10250-10259, 2003.
dc.identifier0022-538X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8521
dc.identifier10.1128/JVI.77.19.10250–10259.2003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869867
dc.descriptionTo evaluate the immunogenicity of human immunodeficiency virus (HIV) type 1 p55gag virus-like particles (VLPs) released by budding from yeast spheroplasts, we have analyzed the effects of yeast VLPs on monocytederived dendritic cells (DCs). Yeast VLPs were efficiently incorporated into DCs via both macropinocytosis and endocytosis mediated by mannose-recognizing receptors, but not the mannose receptor. The uptake of yeast VLPs induced DC maturation and enhanced cytokine production, notably, interleukin-12 p70. We showed that yeast membrane components may contribute to DC maturation partly through Toll-like receptor 2 signaling. Thus, Gag particles encapsulated by yeast membrane may have an advantage in stimulating Gag-specific immune responses. We found that yeast VLPs, but not the control yeast membrane fraction, were able to activate both CD4 and CD8 T cells of HIV-infected individuals. We tested the effect of cross-presentation of VLP by DCs in two subjects recruited into a long-term nonprogressor-slow progressor cohort. When yeast VLP-loaded DCs of these patients were cocultured with peripheral blood mononuclear cells for 7 days, approximately one-third of the Gag-specific CD8 T cells were activated and became perforin positive. However, some of the Gag-specific CD8 T cells appeared to be lost during in vitro culture, especially in a patient with a high virus load. Our results suggest that DCs loaded with yeast VLPs can activate Gag-specific memory CD8 T cells to become effector cells in chronically HIV-infected individuals, but there still remain unresponsive Gag-specific T-cell populations in these patients.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectApresentação do Antígeno
dc.subjectLinfócitos T CD8-Positivos/imunologia
dc.subjectCélulas Dendríticas/fisiologia
dc.subjectProdutos do Gene gag/fisiologia
dc.subjectHIV-1/fisiologia
dc.subjectGlicoproteínas de Membrana/biossíntese
dc.subjectPrecursores de Proteínas/fisiologia
dc.subjectVirion/fisiologia
dc.subjectTécnicas de Cocultura
dc.subjectHumanos
dc.subjectInterferon gama/biossíntese
dc.subjectInterleucina-12/biossíntese
dc.subjectAtivação Linfocitária
dc.subjectGlicoproteínas de Membrana/fisiologia
dc.subjectProteínas Citotóxicas Formadoras de Poros
dc.subjectReceptores de Superfície Celular/fisiologia
dc.subjectSaccharomyces cerevisiae/genética
dc.titleOutbreak of acute Chagas disease occurred in the state of Bahia, Brazil
dc.typeArticle


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