A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters

dc.creatorOliveira, Viviane Klassen de
dc.creatorCamargo, Bruno Dias
dc.creatorAlexandrino, Fabiana
dc.creatorMorello, Luis Gustavo
dc.creatorMarchini, Fabrício Klerynton
dc.creatorAoki, Mateus Nóbrega
dc.creatorBlanes, Lucas
dc.date2021-11-24T17:31:57Z
dc.date2021-11-24T17:31:57Z
dc.date2021
dc.date.accessioned2023-09-26T21:08:32Z
dc.date.available2023-09-26T21:08:32Z
dc.identifierOLIVEIRA, Viviane Klassen de et al. A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters. Biomedical Microdevices, v.23, n.24, p. 1–8, 2021.
dc.identifier1572-8781
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50024
dc.identifier10.1007/s10544-021-00563-2
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869808
dc.descriptionThis article describes the fabrication of a low-cost Polymerase Chain Reaction (PCR) instrument to detect diseases. In order to reduce the instrument price and simplify construction we developed an alternative fabrication process, transforming conventional printed circuit boards (PCB) in heating elements, avoiding the use of aluminum heating/cooling blocks and Peltier devices. To cool down the reaction a simple computer fan was used. The vial holder was fabricated using two double side PCB boards assembled in a sandwich-like confguration. The bottom PCB has a resistance of 0.9 Ω used to heat the reaction mix, while the top layer has a resistance of 1.1 Ω to heat the vial body, preventing vapor condensation. The top board was maintained at~110±1 °C during all cycles. The fnal device was able to heat and cool down the reaction at rates of~2.0 °C/s, a rate comparable to commercial thermocyclers. An SMD NTC thermistor was used as temperature sensors, and a PID (proportional–integral–derivative) control algorithm was implemented to acquire and precisely control the temperature. We also discuss how the instrument is calibrated. The device was tested successfully for the amplifcation of T. pallidum (Syphilis) bacterial DNA and Zika virus RNA samples, showing similar performance to a commercial PCR instrument.
dc.formatapplication/pdf
dc.languagepor
dc.publisherSpringer
dc.rightsopen access
dc.subjectPCR
dc.subjectPolymerase Chain Reaction
dc.subjectReagent Kits, Diagnostic
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectJuego de Reactivos para Diagnóstico
dc.subjectRéaction de polymérisation en chaîne
dc.subjectTrousses de réactifs pour diagnostic
dc.subjectReação em Cadeia da Polimerase
dc.subjectKit de Reagentes para Diagnóstico
dc.titleA low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
dc.typeArticle


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