Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis

dc.creatorNacife, Valéria Pereira
dc.creatorMeirelles, Maria de Nazareth Leal de
dc.creatorSilva Filho, Fernando Costa e
dc.date2020-10-13T13:00:56Z
dc.date2020-10-13T13:00:56Z
dc.date1998
dc.date.accessioned2023-09-26T21:07:48Z
dc.date.available2023-09-26T21:07:48Z
dc.identifierNACIFE, Valéria Pereira; MEIRELLES, Maria de Nazareth Leal de; SILVA FILHO, Fernando Costa e. Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 6, p. 807-814, Nov./Dec. 1998.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43954
dc.identifier10.1590/S0074-02761998000600020
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869627
dc.descriptionThe electrostatic surface charge and surface tension of mononuclear cells/monocytes obtained from young and adult marsupials (Didelphis marsupialis) were investigated by using cationized ferritin and colloidal iron hydroxyde, whole cell electrophoresis, and measurements of contact angles. Anionic sites were found distributed throughout the entire investigated cell surfaces. The results revealed that the anionic character of the cells is given by electrostatic charges corresponding to -18.8 mV (cells from young animals) and -29.3 mV (cells from adult animals). The surface electrostatic charge decreased from 10 to 65.2% after treatment of the cells with each one of trypsin, neuraminidase and phospholipase C. The hydrophobic nature of the mononuclear cell surfaces studied by using the contact angle method revealed that both young and adult cells possess cell surfaces of high hidrofilicity since the angles formed with drops of saline water were 42.5°and 40.8°, respectively. Treatment of the cells with trypsin or neuraminidase rendered their surfaces more hydrophobic, suggesting that sialic acid-containing glycoproteins are responsible for most of the hydrophilicity observed in the mononuclear cell surfaces from D. marsupialis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectDidelphis marsupialis
dc.subjectMonócitos
dc.subjectCarga de superfície
dc.subjectTensão superficial
dc.subjectMonocytes
dc.subjectSurface charge
dc.subjectSurface tension
dc.subjectDidelphis marsupialis
dc.titleBasic Surface Properties of Mononuclear Cells from Didelphis marsupialis
dc.typeArticle


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