MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut

dc.creatorVidal, Vítor Ennes
dc.creatorMenna-Barreto, Rubem F. S.
dc.creatorSantos, André L. S.
dc.creatorBranquinha, Marta H.
dc.creatord`Avila-Levy, Claudia M.
dc.date2016-09-27T12:01:59Z
dc.date2016-09-27T12:01:59Z
dc.date2011
dc.date.accessioned2023-09-26T21:07:36Z
dc.date.available2023-09-26T21:07:36Z
dc.identifierVIDAL, Vitor Ennes; et al. MDL28170, a Calpain Inhibitor, Affects Trypanosoma cruzi Metacyclogenesis, Ultrastructure and Attachment to Rhodnius prolixus Midgut. Plos One, v.6, n.4, e18371, 9p, Apr. 2011.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15956
dc.identifier10.1371/journal.pone.0018371
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869573
dc.descriptionBackground: Trypanosoma cruzi is the etiological agent of Chagas’ disease. During the parasite life cycle, many molecules are involved in the differentiation process and infectivity. Peptidases are relevant for crucial steps of T. cruzi life cycle; as such, it is conceivable that they may participate in the metacyclogenesis and interaction with the invertebrate host. Methodology/Principal Findings: In this paper, we have investigated the effect of the calpain inhibitor MDL28170 on the attachment of T. cruzi epimastigotes to the luminal midgut surface of Rhodnius prolixus, as well as on the metacyclogenesis process and ultrastructure. MDL28170 treatment was capable of significantly reducing the number of bound epimastigotes to the luminal surface midgut of the insect. Once the cross-reactivity of the anti-Dm-calpain was assessed, it was possible to block calpain molecules by the antibody, leading to a significant reduction in the capacity of adhesion to the insect guts by T. cruzi. However, the antibodies were unable to interfere in metacyclogenesis, which was impaired by the calpain inhibitor presenting a significant reduction in the number of metacyclic trypomastigotes. The calpain inhibitor also promoted a direct effect against bloodstream trypomastigotes. Ultrastructural analysis of epimastigotes treated with the calpain inhibitor revealed disorganization in the reservosomes, Golgi and plasma membrane disruption. Conclusions/Significance: The presence of calpain and calpain-like molecules in a wide range of organisms suggests that these proteins could be necessary for basic cellular functions. Herein, we demonstrated the effects of MDL28170 in crucial steps of the T. cruzi life cycle, such as attachment to the insect midgut and metacyclogenesis, as well as in parasite viability and morphology. Together with our previous findings, these results help to shed some light on the functions of T. cruzi calpains. Considering the potential roles of these molecules on the interaction with both invertebrate and vertebrate hosts, it is interesting to improve knowledge on these molecules in T. cruzi.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectDoença de Chagas
dc.subjectCalpaína
dc.subjectTrypanosoma cruzi
dc.subjectChagas Disease
dc.subjectRhodnius prolixus
dc.subjectCalpain Inhibitor
dc.titleMDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
dc.typeArticle


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