Characterization of a monoclonal antibody recognizing mast cells

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorNogueira, T. de Oliveira
dc.creatorAubin, J. E.
dc.date2014-05-27T11:18:16Z
dc.date2016-10-25T18:14:39Z
dc.date2014-05-27T11:18:16Z
dc.date2016-10-25T18:14:39Z
dc.date1997-10-08
dc.date.accessioned2017-04-06T00:50:20Z
dc.date.available2017-04-06T00:50:20Z
dc.identifierHistology and Histopathology, v. 12, n. 4, p. 919-923, 1997.
dc.identifier0213-3911
dc.identifierhttp://hdl.handle.net/11449/65208
dc.identifierhttp://acervodigital.unesp.br/handle/11449/65208
dc.identifierWOS:A1997XX08600004
dc.identifier2-s2.0-0030861549.pdf
dc.identifier2-s2.0-0030861549
dc.identifierhttp://hdl.handle.net/11449/65208
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/886954
dc.descriptionImmunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells.
dc.languageeng
dc.relationHistology and Histopathology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectConnective tissue
dc.subjectHeterogeneity
dc.subjectImmunohistochemistry
dc.subjectIntestine
dc.subjectMast cells
dc.subjectmonoclonal antibody
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectantigen recognition
dc.subjectcalvaria
dc.subjectcontrolled study
dc.subjectfetus
dc.subjectimmunohistochemistry
dc.subjectintestine
dc.subjectmast cell
dc.subjectnonhuman
dc.subjectrat
dc.subjectskin
dc.subjectAnimals
dc.subjectAntibodies, Monoclonal
dc.subjectCells, Cultured
dc.subjectConnective Tissue Cells
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectIntestines
dc.subjectMast Cells
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectRespiratory Muscles
dc.subjectTolonium Chloride
dc.subjectAnimalia
dc.titleCharacterization of a monoclonal antibody recognizing mast cells
dc.typeOtro


Este ítem pertenece a la siguiente institución