dc.creatorGiacoia-Gripp, Carmem Beatriz Wagner
dc.creatorCazote, Andressa da Silva
dc.creatorSilva, Tatiana Pereira da
dc.creatorSant'Anna, Flávia Marinho
dc.creatorSchmaltz, Carolina Arana Stanis
dc.creatorBrum, Tania de Souza
dc.creatorMatos, Juliana Arruda de
dc.creatorSilva, Júlio
dc.creatorBenjamin, Aline
dc.creatorPilotto, José Henrique
dc.creatorRolla, Valeria Cavalcanti
dc.creatorMorgado, Mariza Gonçalves
dc.creatorScott-Algara, Daniel
dc.date2019-12-13T14:59:57Z
dc.date2019-12-13T14:59:57Z
dc.date2019
dc.date.accessioned2023-09-26T21:06:58Z
dc.date.available2023-09-26T21:06:58Z
dc.identifierGIACOIA-GRIPP, Carmem Beatriz Wagner et al. Changes in the NK cell repertoire related to initiation of TB treatment and onset of immune reconstitution inflammatory syndrome in TB/HIV co-infected patients in Rio de Janeiro, Brazil-ANRS 12274. Frontiers in Immunology, v. 10, p. 1-17, Ago. 2019.
dc.identifier1664-3224
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37929
dc.identifier10.3389/fimmu.2019.01800
dc.identifier1664-3224
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869395
dc.descriptionTuberculosis (TB) is the most common comorbidity and the leading cause of death among HIV-infected individuals. Although the combined antiretroviral therapy (cART) during TB treatment improves the survival of TB/HIV patients, the occurrence of immune reconstitution inflammatory syndrome (IRIS) in some patients poses clinical and scientific challenges. This work aimed to evaluate blood innate lymphocytes during therapeutic intervention for both diseases and their implications for the onset of IRIS. Natural killer (NK) cells, invariant NKT cells (iNKT), γδ T cell subsets, and in vitro NK functional activity were characterized by multiparametric flow cytometry in the following groups: 33 TB/HIV patients (four with paradoxical IRIS), 27 TB and 25 HIV mono-infected subjects (prior to initiation of TB treatment and/or cART and during clinical follow-up to 24 weeks), and 25 healthy controls (HC). Concerning the NK cell repertoire, several activation and inhibitory receptors were skewed in the TB/HIV patients compared to those in the other groups, especially the HCs. Significantly higher expression of CD158a (p = 0.025), NKp80 (p = 0.033), and NKG2C (p = 0.0076) receptors was detected in the TB/HIV IRIS patients than in the non-IRIS patients. Although more NK degranulation was observed in the TB/HIV patients than in the other groups, the therapeutic intervention did not alter the frequency during follow-up (weeks 2-24). A higher frequency of the γδ T cell population was observed in the TB/HIV patients with inversion of the Vδ2+/Vδ2- ratio, especially for those presenting pulmonary TB, suggesting an expansion of particular γδ T subsets during TB/HIV co-infection. In conclusion, HIV infection impacts the frequency of circulating NK cells and γδ T cell subsets in TB/HIV patients. Important modifications of the NK cell repertoire were observed after anti-TB treatment (week 2) but not during the cART/TB follow-up (weeks 6-24). An increase of CD161+ NK cells was related to an unfavorable outcome. Despite the low number of cases, a more preserved NK cell profile was detected in IRIS patients previous to treatment, suggesting a role for these cells in IRIS onset. Longitudinal evaluation of the NK repertoire showed the impact of TB treatment and implicated these cells in TB pathogenesis in TB/HIV co-infected patients.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectTuberculosis
dc.subjectHIV
dc.subjectTB/HIV co-infected patients
dc.subjectImmune reconstitution inflammatory
dc.subjectRio de Janeiro
dc.subjectBrazil
dc.titleChanges in the NK cell repertoire related to initiation of TB treatment and onset of immune reconstitution inflammatory syndrome in TB/HIV co-infected patients in Rio de Janeiro, Brazil-ANRS 12274
dc.typeArticle


Este ítem pertenece a la siguiente institución