CD8low T cells expanded following acute Trypanosoma cruzi infection and benznidazole treatment are a relevant subset of IFN-γ producers

dc.creatorSantos, Alessandro Marins dos
dc.creatorOlivieri, Bianca Perdigão
dc.creatorReis, Rafaella Ferreira
dc.creatorMeis, Juliana de
dc.creatorSilva, Andrea Alice
dc.creatorAraujo-Jorge, Tania C. de
dc.creatorVieira, Joseli Lannes
dc.creatorAlmeida, Vinicius Cotta de
dc.date2021-02-08T19:22:23Z
dc.date2021-02-08T19:22:23Z
dc.date2020
dc.date.accessioned2023-09-26T21:06:47Z
dc.date.available2023-09-26T21:06:47Z
dc.identifierSANTOS, Alessandro Marins dos et al. CD8low T cells expanded following acute Trypanosoma cruzi infection and benznidazole treatment are a relevant subset of IFN-γ producers. Plos Negl Trop Dis., v. 14, n. 12, e0008969, 15p, Dec. 2020.
dc.identifier1935-2727
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/45994
dc.identifier10.1371/journal. pntd.0008969
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869346
dc.descriptionCD8 T cells are regarded as pivotal players in both immunoprotection and immunopathology following Trypanosoma cruzi infection. Previously, we demonstrated the expansion of CD8+ T lymphocytes in the spleen of T. cruzi-infected mice under treatment with benznidazole (Nbenzyl- 2-nitroimidazole acetamide; Bz), a drug available for clinical therapy. This finding underlies the concept that the beneficial effects of Bz on controlling acute T. cruzi infection are related to a synergistic process between intrinsic trypanocidal effect and indirect triggering of the active immune response. In the present study, we particularly investigated the effect of Bz treatment on the CD8+ T cell subset following T. cruzi infection. Herein we demonstrated that, during acute T. cruzi infection, Bz treatment reduces and abbreviates the parasitemia, but maintains elevated expansion of CD8+ T cells. Within this subset, a remarkable group of CD8low cells was found in both Bz-treated and non-treated infected mice. In Bz-treated mice, early pathogen control paralleled the lower frequency of recently activated CD8low cells, as ascertained by CD69 expression. However, the CD8low subset sustains significant levels of CD44highCD62Llow and CD62LlowT-bethigh effector memory T cells, in both Bz-treated and non-treated infected mice. These CD8low cells also comprise the main group of spontaneous interferon (IFN)-γ-producing CD8+ T cells. Interestingly, following in vitro anti-CD3/CD28 stimulation, CD8+ T cells from Bz-treated T. cruzi-infected mice exhibited higher frequency of IFN-γ+ cells, which bear mostly a CD8low phenotype. Altogether, our results point to the marked presence of CD8low T cells that arise during acute T. cruzi infection, with Bz treatment promoting their significant expansion along with a potential effector program for high IFN-γ production.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectCélulas CD8low T
dc.subjectInfecção por trypanosoma cruzi
dc.subjectTratamento de benznidazol
dc.subjectProdutores de γ IFN
dc.subjectCD8low T cells
dc.subjectTrypanosoma cruzi infection
dc.subjectBenznidazole Treatment
dc.subjectIFN-γ producers
dc.titleCD8low T cells expanded following acute Trypanosoma cruzi infection and benznidazole treatment are a relevant subset of IFN-γ producers
dc.typeArticle


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