Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells

dc.creatorOliveira, Iara dos Santos da Silva
dc.creatorColares, Aracélio Viana
dc.creatorCardoso, Flávia de Oliveira
dc.creatorTellis, Carla Junqueira Moragas
dc.creatorChagas, Maria do Socorro dos Santos
dc.creatorBehrens, Maria Dutra
dc.creatorCalabrese, Kátia da Silva
dc.creatorSouza, Fernando Almeida
dc.creatorSilva, Ana Lúcia Abreu
dc.date2020-04-23T15:15:30Z
dc.date2020-04-23T15:15:30Z
dc.date2019
dc.date.accessioned2023-09-26T21:06:44Z
dc.date.available2023-09-26T21:06:44Z
dc.identifierOLIVEIRA, Iara dos Santos da Silva et al. Vernonia polysphaera Baker: Antiinflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells. PLoS ONE, v. 14, n. 12, p. 1-19, 12 Dec. 2019.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/40935
dc.identifier10.1371/journal.pone.0225275
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869333
dc.descriptionSpecies of the Vernonia genius are widely distributed across the world. In traditional communities, they are commonly used in popular medicine for the treatment of inflammatory diseases. The objective of the present study was to evaluate the anti-inflammatory activity of Vernonia polysphaera Baker hydroalcoholic extract. A λ-carrageenan-induced paw edema and peritonitis model was established in BALB/c mice. The in vitro activity of the extract was measured on LPS-stimulated RAW 264.7 cells. There was no toxic effect on mice or on the cells treated with the extract. Animals treated with V. polysphaera extract demonstrated inhibition of paw edema in comparison with the untreated animals at all the analyzed doses. In peritonitis, treatment with the extract at a dose of 500 mg/kg resulted in a lower total leukocyte count in the peritoneal fluid and blood and lower levels of IL-1β, IL-6, TNF-α and PGE-2 than the control group. Cells treated with 50 and 100 μg/mL of the extract exhibited lower levels of nitrite and pro-inflammatory cytokine production and lower COX-2, NF-κB expression. The V. polysphaera extract demonstrated an anti-inflammatory effect, interfering with cell migration, reducing pro-inflammatory cytokine levels and COX-2 expression and consequent interference with PGE-2, as well as inhibiting NF-κB transcription.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectVernonia polysphaera Baker
dc.subjectAtividade anti-inflamatória
dc.subjectIn vivo
dc.subjectCélulas RAW 264.7
dc.subjectEfeito inibitório
dc.subjectVernonia polysphaera Baker
dc.subjectAntiinflammatory activity
dc.subjectIn vivo
dc.subjectRAW 264.7 cells
dc.subjectInhibitory effect
dc.titleVernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells
dc.typeArticle


Este ítem pertenece a la siguiente institución