Bismuth-based nanoparticles impair adipogenic differentiation of human adipose-derived mesenchymal stem cells

dc.creatorRibeiro, Annanda Lyra
dc.creatorBassai, Letícia Werzel
dc.creatorRobert, Anny Waloski
dc.creatorMachado, Thiago Neves
dc.creatorBezerra Júnior, Arandi Ginane
dc.creatorHorinouchi, Cintia Delai da Silva
dc.creatorAguiar, Alessandra Melo de
dc.date2021-11-09T18:48:51Z
dc.date2021-11-09T18:48:51Z
dc.date2021
dc.date.accessioned2023-09-26T21:06:31Z
dc.date.available2023-09-26T21:06:31Z
dc.identifierRIBEIRO, Annanda Lyra et al. Bismuth-based nanoparticles impair adipogenic differentiation of human adipose-derived mesenchymal stem cells. Toxicology in Vitro, v. 77, n. 105248, p. 1–10, 2021.
dc.identifier0887-2333
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/49753
dc.identifier10.1016/j.tiv.2021.105248
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869277
dc.descriptionBismuth-based nanoparticles (BiNPs) have attracted attention for their potential biomedical applications. However, there is a lack of information concerning their interaction with biological systems. In this study, it was investigated the effect of physically synthesized BiNPs to human adipose-derived stem cells (ADSCs). We first evaluated the influence of BiNPs on cell viability, cell morphology, mitochondrial function and cell proliferation. Further, the impact of BiNPs on adipogenic differentiation was also explored. Cytotoxicity assays have demonstrated that BiNPs did not reduce relative cell viability of ADSC except at the highest tested concentration (345 μg/ml). Analysis of cell morphology performed by transmission electron microscopy confirmed that BiNPs induced cell damage only at a high concentration (302.24 μg/ml), equivalent to IC50 concentration. Moreover, BiNPs exposure increased the expression of the cell proliferation marker Ki-67 and the incorporation of the thymidine analogue EdU into cell DNA, suggesting that these nanoparticles could be stimulating ADSC proliferation. BiNPs also increased the mitochondrial membrane potential. Furthermore, BiNPs reduced ADSC adipogenic differentiation as measured by lipid droplet accumulation and mRNA expression levels of the specific adipogenesis biomarkers PPARγ, C/EPBɑ and FABP4. Thus, BiNPs affect the nonspecific (viability, proliferation and mitochondrial activity) and specific (adipogenesis) cellular mechanisms of ADSCs.
dc.formatapplication/pdf
dc.languagepor
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectNanoparticles
dc.subjectBismuth
dc.subjectMesenchymal Stem Cells
dc.subjectCell Differentiation
dc.subjectNanopartículas
dc.subjectCélulas Madre Mesenquimatosas
dc.subjectDiferenciación Celular
dc.subjectNanoparticules
dc.subjectCellules souches mésenchymateuses
dc.subjectDifférenciation cellulaire
dc.subjectNanopartículas
dc.subjectBismuto
dc.subjectCélulas-Tronco Mesenquimais
dc.subjectDiferenciação Celular
dc.titleBismuth-based nanoparticles impair adipogenic differentiation of human adipose-derived mesenchymal stem cells
dc.typeArticle


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