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Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorNunes, Caris Maroni
dc.creatorTundisi, Regina Nardini
dc.creatorGarcia, José Fernando
dc.creatorHeinemann, Marcos Brayan
dc.creatorOgassawara, Saemi
dc.creatorRichtzenhain, Leonardo José
dc.date2014-05-27T11:18:15Z
dc.date2016-10-25T18:14:35Z
dc.date2014-05-27T11:18:15Z
dc.date2016-10-25T18:14:35Z
dc.date1997-09-01
dc.date.accessioned2017-04-06T00:50:05Z
dc.date.available2017-04-06T00:50:05Z
dc.identifierRevista do Instituto de Medicina Tropical de Sao Paulo, v. 39, n. 5, p. 253-256, 1997.
dc.identifier0036-4665
dc.identifierhttp://hdl.handle.net/11449/65174
dc.identifierhttp://acervodigital.unesp.br/handle/11449/65174
dc.identifier10.1590/S0036-46651997000500002
dc.identifierS0036-46651997000500002
dc.identifier2-s2.0-0031217069
dc.identifierhttp://dx.doi.org/10.1590/S0036-46651997000500002
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/886923
dc.descriptionVisceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis and A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed.
dc.languageeng
dc.relationRevista do Instituto de Medicina Tropical de São Paulo
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAscaris suum
dc.subjectImmunodiagnosis
dc.subjectToxocara canis antigens
dc.subjectVisceral larva migrans
dc.subjectWestern blotting
dc.subjectparasite antigen
dc.subjectanimal
dc.subjectblood
dc.subjectcross reaction
dc.subjectfemale
dc.subjectimmunology
dc.subjectToxocara canis
dc.subjectvisceral larva migrans syndrome
dc.subjectAnimals
dc.subjectAntigens, Helminth
dc.subjectBlotting, Western
dc.subjectCross Reactions
dc.subjectFemale
dc.subjectLarva Migrans, Visceral
dc.titleCross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
dc.typeOtro


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