Improved rolling circle amplification (RCA) of hepatitis B virus (HBV) relaxed-circular serum DNA (RC-DNA)

dc.creatorMartel, Nora
dc.creatorGomes, Selma A.
dc.creatorChemin, Isabelle
dc.creatorTrépo, Christian
dc.creatorKay, Alan
dc.date2016-07-19T11:24:57Z
dc.date2016-07-19T11:24:57Z
dc.date2013
dc.date.accessioned2023-09-26T21:06:18Z
dc.date.available2023-09-26T21:06:18Z
dc.identifierMARTEL, Nora: et al. Improved rolling circle amplification (RCA) of hepatitis B virus (HBV) relaxed-circular serum DNA (RC-DNA). Journal of Virological Methods, v.193, n.2, p.653-659, Nov. 2013.
dc.identifier0166-0934
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14965
dc.identifier10.1016/j.jviromet.2013.07.045
dc.identifier1879-0984
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869217
dc.descriptionFor functional analysis of HBV isolates, epidemiological studies and correct identification of recombinant genomes, the amplification of complete genomes is necessary. A method for completely in vitro amplification of full-length HBV genomes starting from serum RC-DNA is described. This uses in vitro completion/ligation of plus-strand HBV RC-DNA and amplification using Rolling-Circle Amplification, eventually followed by a genomic PCR. The method can amplify complete HBV genomes from sera with viral loads ranging from >1.0E+8 IU/ml down to 1.0E+3 IU/ml. The method can be applied to archived sera that have undergone long-term storage or to archived DNA serum extracts. The genomes can easily be cloned. HBV genotypes A-G can all be amplified with no apparent problems. A recombinant subgenotype A3/genotype E genome was identified and fully sequenced.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectVírus da Hepatite B
dc.subjectGenótipo
dc.subjectHBV
dc.subjectSerum relaxed-circular DNA
dc.subjectRolling-Circle Amplification (RCA)
dc.subjectHBV genotypes
dc.subjectRecombinant genomes
dc.titleImproved rolling circle amplification (RCA) of hepatitis B virus (HBV) relaxed-circular serum DNA (RC-DNA)
dc.typeArticle


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