Xenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model

dc.creatorYanasse, Ricardo Hideki
dc.creatorLábio, Roger William de
dc.creatorMarques, Leonardo
dc.creatorFukasawa, Josianne Tomazini
dc.creatorSegato, Rosimeire
dc.creatorKinoshita, Angela
dc.creatorMatsumoto, Mariza Akemi
dc.creatorFelisbino, Sergio Luis
dc.creatorSouza, Bruno Solano de Freitas
dc.creatorSantos, Ricardo Ribeiro dos
dc.creatorPayão, Spencer Luiz Marques
dc.date2019-08-07T13:38:37Z
dc.date2019-08-07T13:38:37Z
dc.date2019
dc.date.accessioned2023-09-26T21:05:57Z
dc.date.available2023-09-26T21:05:57Z
dc.identifierYANASSE, Ricardo Hideki et al. Xenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model. Experimental and Therapeutic Medicine, v. 17, p. 4344-4356, 2019.
dc.identifier1792-0981
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/34648
dc.identifier10.3892/etm.2019.7499
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869122
dc.descriptionSantos, Ricardo Ribeiro dos; Souza Bruno Solano de Freitas. Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento.
dc.descriptionSão Paulo Research Foundation (FAPESP) (grant no. 12/21007‑0).
dc.descriptionStem cells in platelet-rich plasma (PRP) scaffolds may be a promising treatment for cartilage repair. Human dental pulp stem cell (hDPSC) subpopulations have been identified to have substantial angiogenic, neurogenic and regenerative potential when compared with other stem cell sources. The present study evaluated the potential of hDPSCs in a PRP scaffold to regenerate full-thickness cartilage defects in rabbits. Full-thickness articular cartilage defects were created in the patellar groove of the femur of 30 rabbits allocated into three experimental groups: Those with an untreated critical defect (CTL), those treated with PRP (PRP) and those treated with stem cells in a PRP scaffold (PRP+SC). The patellar grooves of the femurs from the experimental groups were evaluated macroscopically and histologically at 6 and 12 weeks post-surgery. The synovial membranes were also collected and evaluated for histopathological analysis. The synovial lining cell layer was enlarged in the CTL group compared with the PRP group at 6 weeks (P=0.037) but not with the PRP+SC group. All groups exhibited low-grade synovitis at 6 weeks and no synovitis at 12 weeks. Notably, macroscopic grades for the area of articular cartilage repair for the PRP+SC group were significantly improved compared with those in the CTL (P=0.001) and PRP (P=0.049) groups at 12 weeks. Furthermore, histological scores (modified O'Driscoll scoring system) of the patellar groove articular cartilage in the PRP+SC and PRP groups, in which the articular cartilage was primarily hyaline-like, were significantly higher compared with those in the CTL group at 12 weeks (P=0.002 and P=0.007, respectively). The present results support the therapeutic use of hDPSCs for the treatment of full-thickness articular cartilage defects.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpandidos Publications
dc.rightsrestricted access
dc.subjectCartilagem
dc.subjectPolpa dentária
dc.subjectJoelho
dc.subjectPlasma rico em plaquetas
dc.subjectCélulas estaminais
dc.subjectCartilage
dc.subjectDental pulp
dc.subjectKnee
dc.subjectPlatelet‑rich plasma
dc.subjectStem cells
dc.titleXenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model
dc.typeArticle


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