Brasil | Article
dc.creatorAcosta, Enrique Medina
dc.creatorPaul, Simon
dc.creatorTomlinson, Stephen
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.date2014-09-09T17:16:26Z
dc.date2014-09-09T17:16:26Z
dc.date1994
dc.date.accessioned2023-09-26T21:05:40Z
dc.date.available2023-09-26T21:05:40Z
dc.identifierMEDINA-ACOSTA, E. et al. Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp. Molecular Biochemical Parasitology, v. 64, n. 2, p. 273-282, 1994.
dc.identifier0166-6851
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8335
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869046
dc.descriptionEndotrypanum (order Kinetoplastida: family Trypanosomatidae) is a parasite of forest dwelling tree sloths (Edentata: genera Choleopus and Bradypus). Unique among the haemoflagellates, this protozoan has an intraerythrocytic phase in the mammalian host. Nevertheless, many striking similarities exist between Endotrypanum and the human pathogen Leishmania that make it a useful model for epidemiological and evolutionary aspects of the biology of trypanosomatids. Importantly, Endotrypanum species share both the insect vector and host reservoir with certain species of Leishmania (subgenus Viannia). Because mixed infections with Endotrypanum and Leishmania are common in sloths and, therefore, likely to occur in the sandfly vector, there is a need for adequate biochemical markers to distinguish Endotrypanum from Leishmania infections. In this paper we show that Endotrypanum promastigotes possess sialidase and trans-sialidase activities, which are absent from Leishmania, and which are not closely related to the previously described trypanosomal sialidase/trans-sialidase enzyme. We also document the occurrence in Endotrypanum of homologues of the leishmanial surface metalloproteinase gp63 genes. The combined occurrence of sialidase/trans-sialidase activities and gp63 gene homologues in a unique organism has important ramifications for both field and laboratory studies on the biology of trypanosomatids, especially those related to host infection and evolution
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectEndototrypanum
dc.subjectKinetoplastid
dc.subjectMetalloproteinase
dc.subjectNeuraminidase
dc.subjectTrans-sialidase
dc.subjectSloth
dc.subjectMetaloendopeptidases/genética
dc.subjectNeuraminidase/genética
dc.subjectTrypanosomatina/enzimologia
dc.subjectTrypanosomatina/genética
dc.subjectAnimais
dc.subjectEvolução Biológica
dc.subjectDNA de Protozoário/genética
dc.subjectGenes de Protozoários
dc.subjectGlicoproteínas/genética
dc.subjectLeishmania/enzimologia
dc.subjectLeishmania/genética
dc.subjectHibridização de Ácido Nucleico
dc.subjectEspecificidade da Espécie
dc.subjectTrypanosoma/enzimologia
dc.subjectTrypanosoma/genética
dc.subjectTrypanosomatina/classificação
dc.titleCombined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
dc.typeArticle


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