Systematic Identification and Classification of β-Lactamases Based on Sequence Similarity Criteria: β-Lactamase Annotation

dc.creatorSilveira, Melise Chaves
dc.creatorSilva, Rangeline Azevedo da
dc.creatorMota, Fábio Faria da
dc.creatorCatanho, Marcos
dc.creatorJardim, Rodrigo
dc.creatorGuimarães, Ana Carolina R.
dc.creatorMiranda, Antonio B. de
dc.date2019-02-13T16:27:43Z
dc.date2019-02-13T16:27:43Z
dc.date2018
dc.date.accessioned2023-09-26T21:05:34Z
dc.date.available2023-09-26T21:05:34Z
dc.identifierSILVEIRA, Melise Chaves; et al. Systematic Identification and Classification of β-Lactamases Based on Sequence Similarity Criteria: β-Lactamase Annotation. Evolutionary Bioinformatics, v.14, p.1-11, 2018.
dc.identifier1176-9343
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31608
dc.identifier10.1177/1169343118797351
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869016
dc.descriptionβ-lactamases, the enzymes responsible for resistance to β-lactam antibiotics, are widespread among prokaryotic genera. However, current β-lactamase classification schemes do not represent their present diversity. Here, we propose a workflow to identify and classify βlactamases. Initially, a set of curated sequences was used as a model for the construction of profiles Hidden Markov Models (HMM), specific for each β-lactamase class. An extensive, nonredundant set of β-lactamase sequences was constructed from 7 different resistance proteins databases to test the methodology. The profiles HMM were improved for their specificity and sensitivity and then applied to fully assembled genomes. Five hierarchical classification levels are described, and a new class of β-lactamases with fused domains is proposed. Our profiles HMM provide a better annotation of β-lactamases, with classes and subclasses defined by objective criteria such as sequence similarity. This classification offers a solid base to the elaboration of studies on the diversity, dispersion, prevalence, and evolution of the different classes and subclasses of this critical enzymatic activity.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSAGE Publications
dc.rightsopen access
dc.subjectβ-lactamase
dc.subjectClasse
dc.subjectSub-classe
dc.subjectIdentificação
dc.subjectSemelhança de sequência
dc.subjectβ-lactamase
dc.subjectClass
dc.subjectSubclass
dc.subjectIdentification
dc.subjectSequence similarity
dc.titleSystematic Identification and Classification of β-Lactamases Based on Sequence Similarity Criteria: β-Lactamase Annotation
dc.typeArticle


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