Inhibition of Brazilian ZIKV strain replication in primary human placental chorionic cells and cervical cells treated with nitazoxanide

dc.creatorSouza, Audrien A. A. de
dc.creatorTorres, Lauana R.
dc.creatorLima, Lyana R. P.
dc.creatorPaula, Vanessa de
dc.creatorBarros, José J.
dc.creatorBonecini-Almeida, Maria da Gloria
dc.creatorWaghabi, Mariana Caldas
dc.creatorGardel, Marcelo A.
dc.creatorMeuser-Batista, Marcelo
dc.creatorSouza, Elen M. de
dc.date2020-10-27T19:05:49Z
dc.date2020-10-27T19:05:49Z
dc.date2020
dc.date.accessioned2023-09-26T21:04:52Z
dc.date.available2023-09-26T21:04:52Z
dc.identifierSOUZA, Audrien A. A. de et a. Inhibition of Brazilian ZIKV strain replication in primary human placental chorionic cells and cervical cells treated with nitazoxanide. Brazilian Journal of Infectious Diseases, p. 1-12, Sept. 2020.
dc.identifier1413-8670
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44238
dc.identifier10.1016/j.bjid.2020.09.001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8868828
dc.descriptionZika virus (ZIKV) infection during pregnancy is associated with a congenital syndrome. Although the virus can be detected in human placental tissue and sexual transmission has been verified, it is not clear how the virus reaches the fetus. Despite the emerging severity caused by ZIKV infection, no specific prophylactic and/or therapeutic treatment is available. The aim of the present study was to evaluate the effectiveness antiviral of nitazoxanide (NTZ) in two important congenital transmission targets: (i) a primary culture of human placental chorionic cells, and (ii) human cervical epithelial cells (C33-A) infected with Brazilian ZIKV strain. Initially, NTZ activity was screened in ZIKV infected Vero cells under different treatment regimens with non-toxic drug concentrations for 48 h. Antiviral effect was found only when the treatment was carried out after the viral inoculum. A strong effect against the dengue virus serotype 2 (DENV-2) was also observed suggesting the possibility of treating other Flaviviruses. Additionally, it was shown that the treatment did not reduce the production of infectious viruses in insect cells (C6/36) infected with ZIKV, indicating that the activity of this drug is also related to host factors. Importantly, we demonstrated that NTZ treatment in chorionic and cervical cells caused a reduction of infected cells in a dose-dependent manner and decreased viral loads in up to 2 logs. Pre-clinical in vitro testing evidenced excellent therapeutic response of infected chorionic and cervical cells and point to future NTZ activity investigation in ZIKV congenital transmission models with the perspective of possible repurposing of NTZ to treat Zika fever, especially in pregnant women.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectAntiviral effect
dc.subjectZIKV
dc.subjectRepurposing
dc.subjectPrimary culture
dc.subjectPlacenta
dc.subjectNitazoxanide
dc.titleInhibition of Brazilian ZIKV strain replication in primary human placental chorionic cells and cervical cells treated with nitazoxanide
dc.typeArticle


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