Mast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2

dc.creatorMatos, Natália Alves de
dc.creatorSilva, Josiane Fernandes da
dc.creatorMatsui, Tamires Cardoso
dc.creatorDamasceno, Karine Araujo
dc.creatorDuarte, Igor Dimitri Gama
dc.creatorLemos, Virgínia Soares
dc.creatorCassali, Geovanni D
dc.creatorKlein, André
dc.date2018-06-13T17:32:32Z
dc.date2018-06-13T17:32:32Z
dc.date2013
dc.date.accessioned2023-09-26T21:04:27Z
dc.date.available2023-09-26T21:04:27Z
dc.identifierMast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2
dc.identifier0360-3997
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26877
dc.identifier10.1007/s10753-013-9664-5
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8868713
dc.descriptionDamasceno, Karine Araújo. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
dc.descriptionConselho Nacional de Pesquisa e Desenvolvimento (CNPq/Brazil), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG/Brazil) and CAPES/Brazil.
dc.descriptionProteinase-activated receptor (PAR) 2 has been implicated in eosinophil migration. Mast cell (MC) tryptase has been similarly implicated in allergic diseases through the activation of PAR-2, but the role of this receptor in MC tryptase-induced inflammation is not well elucidated. This study aims to investigate the ability of MC tryptase or PAR-2 activating peptide (SLIGRL-NH2) to induce eosinophil recruitment to the pleural cavity of mice. Mast cell tryptase-injected mice were pretreated with PAR-2 antagonist ENMD-1068. Mice injected with SLIGRL-NH2 were pretreated with mast cell tryptase inhibitor APC 366, and eosinophil migration into the pleural cavity and PAR-2 expression was analyzed after 24 or 48 h. SLIGRL-NH2-induced eosinophil recruitment was inhibited by APC 366, and MC tryptase-induced eosinophil recruitment was abolished by ENMD-1068. MC tryptase induced PAR-2 expression on pleural eosinophils. Our results demonstrate a key role for PAR-2 in mediating eosinophil recruitment in MC tryptase-induced pleurisy in mice. The ability of MC tryptase to inducing PAR-2 expression on eosinophils corroborates the relevance of MC tryptase and PAR-2 on modulating eosinophil migration.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag
dc.rightsopen access
dc.subjectEosinófilos
dc.subjectMigração de eosinófilos
dc.subjectTriptase de mastócitos
dc.subjectPAR-2
dc.subjectPleurisia
dc.subjectEosinophils
dc.subjectEosinophil migration
dc.subjectMast cell tryptase
dc.subjectPAR-2
dc.subjectPleurisy
dc.titleMast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2
dc.typeArticle


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