Histopathological and ultrastructural effects of d-endotoxins of Bacillus thuringiensis serovar israelensis in the midgut of Simulium pertinax larvae (Diptera, Simuliidae)

dc.creatorCavados, C. F. G.
dc.creatorMajerowicz, S.
dc.creatorChaves, J. Q,
dc.creatorCoutinho, C. J. P. C. Araújo
dc.creatorRabinovitch, L.
dc.date2020-11-18T21:11:06Z
dc.date2020-11-18T21:11:06Z
dc.date2004
dc.date.accessioned2023-09-26T21:04:19Z
dc.date.available2023-09-26T21:04:19Z
dc.identifierCAVADOS, C. F. G. et al. Histopathological and ultrastructural effects of d-endotoxins of Bacillus thuringiensis serovar israelensis in the midgut of Simulium pertinax larvae (Diptera, Simuliidae). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 5, p. 493-498, Aug. 2004.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44532
dc.identifier10.1590/S0074-02762004000500006
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8868678
dc.descriptionThe bacterium Bacillus thuringiensis (Bt) produces parasporal crystals containing δ-endotoxins responsible for selective insecticidal activity on larvae. Upon ingestion, these crystals are solubilized in the midgut lumen and converted into active toxins that bind to receptors present on the microvilli causing serious damage to the epithelial columnar cells. We investigated the effect of these endotoxins on larvae of the Simulium pertinax, a common black fly in Brazil, using several concentrations during 4 h of the serovar israelensis strain IPS-82 (LFB-FIOCRUZ 584), serotype H-14 type strain of the Institute Pasteur, Paris. Light and electron microscope observations revealed, by time and endotoxin concentration, increasing damages of the larvae midgut epithelium. The most characteristic effects were midgut columnar cell vacuolization, microvilli damages, epithelium cell contents passing into the midgut lumen and finally the cell death. This article is the first report of the histopathological effects of the Bti endotoxins in the midgut of S. pertinax larvae and the data obtained may contribute to a better understanding of the mode of action of this bacterial strain used as bioinsecticide against black fly larvae.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectSimulium pertinax
dc.subjectUltraestrutura
dc.subjectHistopatologia
dc.subjectBacillus thuringiensis serovar israelensis
dc.subjectSimulium pertinax
dc.subjectMidgut
dc.subjectHistopathology
dc.subjectUltrastructure
dc.titleHistopathological and ultrastructural effects of d-endotoxins of Bacillus thuringiensis serovar israelensis in the midgut of Simulium pertinax larvae (Diptera, Simuliidae)
dc.typeArticle


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