Effect of Selective Phosphodiesterase Inhibitors on the Rat Eosinophil Chemotactic Response In Vitro

dc.creatorAlves, Alessandra C.
dc.creatorPires, Ana Lucia A.
dc.creatorLagente, Vicente
dc.creatorCordeiro, Renato S. B.
dc.creatorMartins, Marco A.
dc.creatorSilva, Patricia M. R. e
dc.date2020-10-10T13:00:45Z
dc.date2020-10-10T13:00:45Z
dc.date1997
dc.date.accessioned2023-09-26T21:04:07Z
dc.date.available2023-09-26T21:04:07Z
dc.identifierALVES, Alessandra C. et al. Effect of Selective Phosphodiesterase Inhibitors on the Rat Eosinophil Chemotactic Response In Vitro, Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, Suppl. 2, p. 201-204, 1997.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43915
dc.identifier10.1590/S0074-02761997000800027
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8868627
dc.descriptionIn the present study, we have performed a comparative analysis of the effect of selective inhibitors of phosphodiesterase (PDE) type III, IV and V on eosinophil chemotaxis triggered by platelet activating factor (PAF) and leukotriene B4 (LTB4) in vitro. The effect of the analogues N6-2'-O-dibutyryladenosine 3':5' cyclic monophosphate (Bt2 cyclic AMP) and N2-2'-O- dibutyrylguanosine 3':5' cyclic monophosphate (Bt2 cyclic GMP) has also been determined. The eosinophils were obtained from the peritoneal cavity of naive Wistar rats and purified in discontinuous Percoll gradients to 85-95% purity. We observed that pre-incubation of eosinophils with the PDE type IV inhibitor rolipram suppressed the chemotactic response triggered by PAF and LTB4, in association with an increase in the intracellular levels of cyclic AMP. In contrast, neither zaprinast (type V inhibitor) nor type III inhibitors milrinone and SK&F 94836 affected the eosinophil migration. Only at the highest concentration tested did the analogue Bt2 cyclic AMP suppress the eosinophil chemotaxis, under conditions where Bt2 cyclic GMP was ineffective. We have concluded that inhibition of PDE IV, but not PDE III or V, was able to block the eosinophil chemotaxis in vitro, suggesting that the suppressive activity of selective PDE IV inhibitors on tissue eosinophil accumulation may, at least, be partially dependent on their ability to directly inhibit the eosinophil migration.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectEosinophil
dc.subjectInibidores de fosfodiesterase
dc.subjectMigração
dc.subjectEosinophil
dc.subjectMigration
dc.subjectPhosphodiesterase inhibitors
dc.titleEffect of Selective Phosphodiesterase Inhibitors on the Rat Eosinophil Chemotactic Response In Vitro
dc.typeArticle


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