Diagnostic application of sensitive and specific phage-exposed epitopes for visceral leishmaniasis and human immunodeficiency virus coinfection

dc.creatorRamos, Fernanda F
dc.creatorTavares, Grasiele S V
dc.creatorLudolf, Fernanda
dc.creatorMachado, Amanda S
dc.creatorSantos, Thaís T O
dc.creatorGonçalves, Isabela A P
dc.creatorDias, Ana C S
dc.creatorAlves, Patrícia T
dc.creatorFraga, Vanessa G
dc.creatorBandeira, Raquel S
dc.creatorOliveira-da-Silva, João A
dc.creatorReis, Thiago A R
dc.creatorLage, Daniela P
dc.creatorMartins, Vívian T
dc.creatorFreitas, Camila S
dc.creatorChaves, Ana Thereza
dc.creatorGuimarães, Nathalia S
dc.creatorChávez-Fumagalli, Miguel A
dc.creatorTupinambás, Unaí
dc.creatorRocha, Manoel O C
dc.creatorCota, Gláucia Fernandes
dc.creatorFujiwara, Ricardo Toshio
dc.creatorBueno, Lílian L
dc.creatorGoulart, Luiz Ricardo
dc.creatorCoelho, Eduardo A F
dc.date2022-06-29T17:05:12Z
dc.date2022-06-29T17:05:12Z
dc.date2021
dc.date.accessioned2023-09-26T21:03:51Z
dc.date.available2023-09-26T21:03:51Z
dc.identifierRAMOS, Fernanda F et al. Diagnostic application of sensitive and specific phage-exposed epitopes for visceral leishmaniasis and human immunodeficiency virus coinfection. Parasitology, v. 148, n. 13, p. 1706-1714, 2021. doi: 10.1017/S0031182021001505
dc.identifier0031-1820
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/53579
dc.identifier10.1017/S0031182021001505
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8868552
dc.descriptionThe diagnosis of visceral leishmaniasis (VL) has improved with the search of novel antigens; however, their performance is limited when samples from VL/human immunodeficiency virus (HIV)-coinfected patients are tested. In this context, studies conducted to identify more suitable antigens to detect both VL and VL/HIC coinfection cases should be performed. In the current study, phage display was performed using serum samples from healthy subjects and VL, HIV-infected and VL/HIV-coinfected patients; aiming to identify novel phage-exposed epitopes to be evaluated with this diagnostic purpose. Nine non-repetitive and valid sequences were identified, synthetized and tested as peptides in enzyme-linked immunosorbent assay experiments. Results showed that three (Pep2, Pep3 and Pep4) peptides showed excellent performance to diagnose VL and VL/HIV coinfection, with 100% sensitivity and specificity values. The other peptides showed sensitivity varying from 50.9 to 80.0%, as well as specificity ranging from 60.0 to 95.6%. Pep2, Pep3 and Pep4 also showed a potential prognostic effect, since specific serological reactivity was significantly decreased after patient treatment. Bioinformatics assays indicated that Leishmania trypanothione reductase protein was predicted to contain these three conformational epitopes. In conclusion, data suggest that Pep2, Pep3 and Pep4 could be tested for the diagnosis of VL and VL/HIV coinfection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherCambridge University Press
dc.rightsrestricted access
dc.subjectDiagnosis; VL/HIV coinfection
dc.subjecthuman immunodeficiency virus
dc.subjectphage display
dc.subjecttrypanothione reductase
dc.subjectvisceral leishmaniasis
dc.titleDiagnostic application of sensitive and specific phage-exposed epitopes for visceral leishmaniasis and human immunodeficiency virus coinfection
dc.typeArticle


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