Synergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCrepaldi-Alves, S. C.
dc.creatorCarneiro, E. M.
dc.creatorBosqueiro, José Roberto
dc.creatorBoschero, A. C.
dc.date2014-05-27T11:18:12Z
dc.date2016-10-25T18:14:21Z
dc.date2014-05-27T11:18:12Z
dc.date2016-10-25T18:14:21Z
dc.date1997-03-01
dc.date.accessioned2017-04-06T00:49:15Z
dc.date.available2017-04-06T00:49:15Z
dc.identifierBrazilian Journal of Medical and Biological Research, v. 30, n. 3, p. 359-361, 1997.
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/11449/65057
dc.identifierhttp://acervodigital.unesp.br/handle/11449/65057
dc.identifier10.1590/S0100-879X1997000300008
dc.identifierS0100-879X1997000300008
dc.identifierWOS:A1997WQ16600008
dc.identifier2-s2.0-0031089124.pdf
dc.identifier2-s2.0-0031089124
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X1997000300008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/886816
dc.descriptionWe studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.8 mM glucose) was similar in control and PRL-treated islets (1.84 ± 0.06% and 2.08 ± 0.07% of the islet insulin content, respectively). At 5.6 and 22 mM glucose, insulin secretion was significantly higher in PRL-treated than in control islets, achieving 1.38 ± 0.15% and 3.09 ± 0.21 % of the islet insulin content in control and 2.43 ± 0.16% and 4.31 ± 0.24% of the islet insulin content in PRL-treated islets, respectively. The expression of the glucose transporter GLUT2 in B-cell membranes was dose-dependently increased by exposure of the islet to increasing glucose concentrations. This effect was potentiated in islets cultured for 7 days in the presence of 2 μg/ml PRL. At 5.6 and 10 mM glucose, the increase in GLUT2 expression in PRL-treated islets was 75% and 150% higher than that registered in the respective control. The data presented here indicate that insulin secretion, induced by different concentrations of glucose, correlates well with the expression of the B-cell-specific glucose transporter GLUT2 in pancreatic islets.
dc.languageeng
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGlucose
dc.subjectGlucose transporter
dc.subjectPancreatic islets
dc.subjectProlactin
dc.subjectglucose
dc.subjectglucose transporter
dc.subjectglucose transporter 2
dc.subjectprolactin
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectculture technique
dc.subjectmetabolism
dc.subjectpancreas islet
dc.subjectphysiology
dc.subjectrat
dc.subjectAnimals
dc.subjectCell Culture Techniques
dc.subjectGlucose Transporter Type 2
dc.subjectIslets of Langerhans
dc.subjectMonosaccharide Transport Proteins
dc.subjectRats
dc.titleSynergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets
dc.typeOtro


Este ítem pertenece a la siguiente institución