A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases

dc.creatorAlves, Carlos Roberto
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.creatorSouza, André Luis Almeida
dc.creatorDe Simone, Salvatore Giovanni
dc.date2017-07-25T13:16:34Z
dc.date2017-07-25T13:16:34Z
dc.date2001
dc.date.accessioned2023-09-26T21:02:10Z
dc.date.available2023-09-26T21:02:10Z
dc.identifierALVES, Carlos Roberto et al. A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases. Cytobios, v. 104, p. 33-41, 2001.
dc.identifier0011-4529
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20388
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8868095
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do estado do Rio de Janeiro (FAPERJ) and FIOCRUZ.
dc.descriptionIn this study computational analysis was used to compile sequence alignments, constructo a dendrogram and calculate physical data in order to predict potential T-cell epitopes of the Leishmania cysteine proteinase. Using multiple alignment of human and Leishmania proteinase sequences deposited on data bank sequences, it was possible to predict that the extreme C-terminus of cysteine proteinase (Cyspep, 355-444) contained three peptides (pl 361-370, pll 415-422 and plll 431-444) with charge score, hydrophobicity and isoelectric points compatible for human leucocyte-associated antigen (HLA) class II binding. The prediction was confirmed in vitro through the ability of synthetic peptides corresponding to the predicted regions to stimulate peripheral blood mononuclear cells of patients with leishmaniasis.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectLeishmania
dc.subjectCisteina proteinases
dc.subjectEpitopos
dc.subjectLeishmania braziliensis
dc.subjectHumanos
dc.subjectLeishmania
dc.subjectCysteine proteinases
dc.subjectEpitopes
dc.subjectLeishmania braziliensis
dc.subjectHumans
dc.titleA strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
dc.typeArticle


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