Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model

dc.creatorNasaré, Alex M.
dc.creatorTedesco, Roberto C.
dc.creatorCristovam, Priscila C.
dc.creatorCenedese, Marcos A.
dc.creatorGalisteo Jr., Andrés J.
dc.creatorAndrade Jr., Heitor F.
dc.creatorGomes, José Álvaro P.
dc.creatorGuimarães, Érik V.
dc.creatorBarbosa, Helene S.
dc.creatorAlonso, Luis G.
dc.date2016-05-19T16:11:15Z
dc.date2016-05-19T16:11:15Z
dc.date2015
dc.date.accessioned2023-09-26T21:01:25Z
dc.date.available2023-09-26T21:01:25Z
dc.identifierNASARÉ, Alex M. et al. Toxoplasma Gondii Infection of Chicken Embryos Causes Retinal Changes and Modulates HSP90B1 Gene Expression: A Promising Ocular Toxoplasmosis Model. Eur J Microbiol Immunol (Bp), v.5, n.4, p.316-20, Nov. 2015.
dc.identifier2062-8633
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14397
dc.identifier10.1556/1886.2015.00024
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8867888
dc.descriptionHSP90B1 is a gene that codifies heat shock protein 108 (HSP108) that belongs to a group of proteins induced under stress situation, and it has close relation with the nervous system, especially in the retina. Toxoplasma gondii causes ocular toxoplasmosis that has been associated with a late manifestation of the congenital toxoplasmosis although experimental models show that morphological alterations are already present during embryological development. Here, we used 18 eyes of Gallus domesticus embryos in 7th and 20th embryonic days to establish a model of congenital ocular toxoplasmosis, experimentally infected in its fifth day correlating with HSP90B1 gene expression. Embryos' eyes were histologically evaluated, and gene expression was performed by real-time polymerase chain reaction (PCR). Our data showed parasite present in the choroid, unusual migration of retinal pigment epithelium, and chorioretinal scars, and a tendency to a lower expression of the HSP90B1 gene upon experimental infection. This is a promising model to better understand T. gondii etiopathogeny.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectDesenvolvimento embrionário
dc.subjectModelos experimentais
dc.subjectToxoplasmose ocular
dc.subjectInfecções oculares parasitárias
dc.subjectRetina
dc.subjectEmbryo development
dc.subjectExperimental models
dc.subjectOcular toxoplasmosis
dc.subjectParasitic ocular infections
dc.subjectRegulation of gene expression in developmen
dc.subjectRetina
dc.titleToxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
dc.typeArticle


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