Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.

dc.creatorFiuza, Jacqueline Araújo
dc.creatorSantiago, Helton da Costa
dc.creatorGannavaram, Angamuthu Selvapandiyane Sreenivas
dc.creatorRicci, Natasha Delaqua
dc.creatorBueno, Lilian Lacerda
dc.creatorBartholomeu, Daniella Castanheira
dc.creatorOliveira, Rodrigo Correa de
dc.creatorNakhasi, Hira Lal
dc.creatorFujiwara, Ricardo Toshio
dc.date2018-12-21T18:18:47Z
dc.date2018-12-21T18:18:47Z
dc.date2013
dc.date.accessioned2023-09-26T21:01:16Z
dc.date.available2023-09-26T21:01:16Z
dc.identifierFIUZA, Jacqueline Araújo et al. Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs. Vaccine., v. 31, n. 14, p. 1785-1792, 2013
dc.identifier0264-410X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30738
dc.identifier10.1016/j.vaccine.2013.01.048
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8867844
dc.descriptionZoonotic visceral leishmaniasis, caused by the intracellular protozoan parasite Leishmania infantum, is a neglected tropical disease that is often fatal when untreated. Dogs are considered the main reservoir of L. infantum in zoonotic VL as the presence of infected dogs may increase the risk for human infection. Canine visceral leishmaniasis (CVL) is a major veterinary and public health problem in Southern Europe, Middle East and South America. Control of animal reservoirs relies on elimination of seropositive dogs in endemic areas. However, treatment of infected dogs is not considered a favorable approach as this can lead to emergence of drug resistance since the same drugs are used to treat human infections. Therefore, vaccination against CVL remains the best alternative in control of the animal reservoirs. In this study, we present data on the immunogenicity profile of a live attenuated parasite LdCen(-/-) in a canine infection model and compared it to that of Leishmune(®), a commercially available recombinant vaccine. The immunogenicity of the LdCen(-/-) parasites was evaluated by antibody secretion, production of intracytoplasmic and secreted cytokines, activation and proliferation of T cells. Vaccination with LdCen(-/-) resulted in high immunogenicity as revealed by the higher IgGTotal, IgG1, and IgG2 production and higher lymphoproliferative response. Further, LdCen(-/-) vaccinated dogs showed higher frequencies of activated CD4+ and CD8+ T cells, IFN-γ production by CD8+ T cells, increased secretion of TNF-α and IL-12/IL-23p40 and decreased secretion of IL-4. These results contribute to the understanding of immunogenicity elicited by live attenuated L. donovani parasites and, consequently, to the development of effective vaccines against visceral leishmaniasis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Science
dc.rightsopen access
dc.subjectLeishmaniose visceral
dc.subjectvisceral leishmaniasis
dc.titleInduction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
dc.typeArticle


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