Metagenomic Analysis of Upwelling-Affected Brazilian Coastal Seawater Reveals Sequence Domains of Type I PKS and Modular NRPS

dc.creatorCuadrat, Rafael R. C.
dc.creatorCury, Juliano C.
dc.creatorDávila, Alberto M. R.
dc.date2016-04-18T13:08:57Z
dc.date2016-04-18T13:08:57Z
dc.date2015
dc.date.accessioned2023-09-26T21:00:30Z
dc.date.available2023-09-26T21:00:30Z
dc.identifierCUADRAT, Rafael R. C.; CURY, Juliano C.; DÁVILA, Alberto M. R. Metagenomic Analysis of Upwelling-Affected Brazilian Coastal Seawater Reveals Sequence Domains of Type I PKS and Modular NRPS. International Journal of Molecular Sciences, v.16, p.28285-28295, 2015.
dc.identifier1661-6596
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/13842
dc.identifier10.3390/ijms161226101
dc.identifier1422-0067
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8867632
dc.descriptionMarine environments harbor a wide range of microorganisms from the three domains of life. These microorganisms have great potential to enable discovery of new enzymes and bioactive compounds for industrial use. However, only ~1% of microorganisms from the environment can currently be identified through cultured isolates, limiting the discovery of new compounds. To overcome this limitation, a metagenomics approach has been widely adopted for biodiversity studies on samples from marine environments. In this study, we screened metagenomes in order to estimate the potential for new natural compound synthesis mediated by diversity in the Polyketide Synthase (PKS) and Nonribosomal Peptide Synthetase (NRPS) genes. The samples were collected from the Praia dos Anjos (Angel’s Beach) surface water—Arraial do Cabo (Rio de Janeiro state, Brazil), an environment affected by upwelling. In order to evaluate the potential for screening natural products in Arraial do Cabo samples, we used KS (keto-synthase) and C (condensation) domains (from PKS and NRPS, respectively) to build Hidden Markov Models (HMM) models. From both samples, a total of 84 KS and 46 C novel domain sequences were obtained, showing the potential of this environment for the discovery of new genes of biotechnological interest. These domains were classified by phylogenetic analysis and this was the first study conducted to screen PKS and NRPS genes in an upwelling affected sample.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectMeio ambiente costeiro
dc.subjectPKS
dc.subjectNRPS
dc.subjectMetagenomics
dc.subjectEnvironmental genomics
dc.subjectUpwelling
dc.subjectCoastal environment
dc.subjectMetagenômica
dc.subjectSurgência
dc.subjectGenômica ambiental
dc.titleMetagenomic Analysis of Upwelling-Affected Brazilian Coastal Seawater Reveals Sequence Domains of Type I PKS and Modular NRPS
dc.typeArticle


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