Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains

dc.creatorSequeira, Patrícia Carvalho de
dc.creatorFonseca, Leila de Souza
dc.creatorSilva, Marlei Gomes da
dc.creatorSaad, Maria Helena Féres
dc.date2020-11-20T20:04:14Z
dc.date2020-11-20T20:04:14Z
dc.date2005
dc.date.accessioned2023-09-26T21:00:19Z
dc.date.available2023-09-26T21:00:19Z
dc.identifierSEQUEIRA. Patrícia Carvalho de et al. Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, p. 743-748, Nov. 2005.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44564
dc.identifier10.1590/S0074-02762005000700012
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8867580
dc.descriptionSimple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectMycobacterium avium
dc.subjectAIDS
dc.subjectMicobactérias não Tuberculosas
dc.subjectReação em Cadeia da Polimerase
dc.subjectPolimorfismo de Fragmento de Restrição
dc.subjectIS1254-restriction fragment length polymorphism
dc.subjectMycobacterium avium
dc.subjectMaDRE-polymerase chain reaction
dc.subjectNontuberculous mycobacteria
dc.subjectAIDS
dc.subjectMicobactérias não Tuberculosas
dc.subjectCélulas-Tronco
dc.subjectPolimorfismo de Fragmento de Restrição
dc.titleMycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains
dc.typeArticle


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