HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)

dc.creatorFreitas, Maria Audilene de
dc.creatorCruz, Rafael Pereira da
dc.creatorSantos, Antonia Thassya Lucas dos
dc.creatorBezerra, José Weverton Almeida
dc.creatorMachado, Antonio Júdson Targino
dc.creatorSantos, Joycy Francely Sampaio dos
dc.creatorRocha, Janaina Esmeraldo
dc.creatorBoligon, Aline Augusti
dc.creatorBezerra, Camila Fonseca
dc.creatorFreitas, Thiago Sampaio de
dc.creatorSilva, Maria Karollyna do Nascimento
dc.creatorMendonça, Ana Cleide Alcântara Morais
dc.creatorCosta, José Galberto Martins da
dc.creatorCoutinho, Henrique Douglas Melo
dc.creatorCunha, Francisco Assis Bezerra da
dc.creatorRibeiro Filho, Jaime
dc.creatorBraga, Maria Flaviana Bezerra Morais
dc.date2022-03-11T13:40:54Z
dc.date2022-03-11T13:40:54Z
dc.date2022
dc.date.accessioned2023-09-26T20:59:22Z
dc.date.available2023-09-26T20:59:22Z
dc.identifierFreitas, Maria Audilene de et al. HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae). 3 Biotech, v. 12, n. 1, p. 1-16, 2022.
dc.identifier2190-5738
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51683
dc.identifierdoi.org/10.1007/s13205-022-03126-1
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8867344
dc.descriptionUniversidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Federal de Santa Maria. Santa Maria, RS, Brasil. Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil.
dc.descriptionFundação Cearense de Apoio ao Desenvolvimento (FUNCAP). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Financiadora de Estudos e Projetos (FINEP).
dc.descriptionSpondias mombin is used in the folk medicine for the treatment of diarrhea and dysentery, indicating that extracts obtained from this species may present pharmacological activities against pathogenic microorganisms. The purpose of this work was to investigate the chemical composition and evaluate the antimicrobial activity of extracts obtained from the leaves (aqueous) and bark (hydroethanolic) of S. mombin both as single treatments and in combination with conventional drugs. Following a qualitative chemical prospection, the extracts were analyzed by HPLC–DAD. The antimicrobial activities were evaluated by microdilution. The combined activity of drugs and extracts was verified by adding a subinhibitory concentration of the extract in the presence of variable drug concentrations. The Minimum Fungicidal Concentration (MFC) was determined by a subculture of the microdilution test, while the effect of the in vitro treatments on morphological transition was analyzed by subculture in moist chambers. While the qualitative analysis detected the presence of phenols and flavonoids, the HPLC analysis identified quercetin, caffeic acid, and catechin as major components in the leaf extract, whereas kaempferol and quercetin were found as major compounds in the bark extract. The extracts showed effective antibacterial activities only against the Gram-negative strains. With regard to the combined activity, the leaf extract potentiated the action of gentamicin and imipenem (against Staphylococcus aureus), while the bark extract potentiated the effect of norfloxacin (against S. aureus), imipenem (against Escherichia coli), and norfloxacin (against Pseudomonas aeruginosa). A more significant antifungal (fungistatic) effect was achieved with the bark extract (even though at high concentrations), which further enhanced the activity of fluconazole. The extracts also inhibited the emission of filaments by Candida albicans and Candida tropicalis. Together, these findings suggest that that the extract constituents may act by favoring the permeability of microbial cells to conventional drugs, as well as by affecting virulence mechanisms in Candida strains.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer
dc.rightsopen access
dc.subjectCompostos fitoquímicos
dc.subjectAntibacterianos
dc.subjectAntifúngicos
dc.subjectPhytochemicals
dc.subjectAnti-Bacterial agents
dc.subjectAntifungal agents
dc.subjectFitoquímicos
dc.subjectAntibacterianos
dc.subjectAntifúngicos
dc.subjectComposés phytochimiques
dc.subjectAntibactériens
dc.subjectAntifongiques
dc.titleHPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
dc.typeArticle


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