Determination of Oxalate in Grass by FIA with a Spectrophotometric Detection System Using a Two Enzyme Reactor

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFernandes, João Roberto
dc.creatorDe Oliveira Neto, Graciliano
dc.creatorTubino, Matthieu
dc.creatorKubota, Lauro T.
dc.date2014-05-27T11:18:09Z
dc.date2016-10-25T18:14:08Z
dc.date2014-05-27T11:18:09Z
dc.date2016-10-25T18:14:08Z
dc.date1996-12-01
dc.date.accessioned2017-04-06T00:48:21Z
dc.date.available2017-04-06T00:48:21Z
dc.identifierAnalytical Sciences, v. 12, n. 3, p. 443-447, 1996.
dc.identifier0910-6340
dc.identifierhttp://hdl.handle.net/11449/64933
dc.identifierhttp://acervodigital.unesp.br/handle/11449/64933
dc.identifier10.2116/analsci.12.443
dc.identifier2-s2.0-0030510001.pdf
dc.identifier2-s2.0-0030510001
dc.identifierhttp://dx.doi.org/10.2116/analsci.12.443
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/886702
dc.descriptionA new methodology for soluble oxalic acid determination in grass samples was developed using a two enzyme reactor in an FIA system. The reactor consisted of 3 U of oxalate oxidase and 100 U of peroxidase immobilized on Sorghum vulgare seeds activated with glutaraldehyde. The carbon dioxide was monitored spectrophotometrically, after reacting with an acid-base indicator (Bromocresol Purple) after it permeated through a PTFE membrane. A linear response range was observed between 0.25 and 1.00mmol l-1 of oxalic acid; the data was fit by the equation A=-0.8(±1.5)+ 57.2(±2.5)[oxalate], with a correlation coefficient of 0.9971 and a relative standard deviation of 2% for n=5. The variance for a 0.25 mmol l-1 oxalic acid standard solution was lower than 4% for 11 measurements. The FIA system allows analysis of 20 samples per hour without prior treatment. The proposed method showed a good correlation with that of the Sigma Kit.
dc.languageeng
dc.relationAnalytical Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGrass
dc.subjectOxalate determination
dc.subjectOxalate oxidase
dc.subjectSorghum vulgare seeds
dc.titleDetermination of Oxalate in Grass by FIA with a Spectrophotometric Detection System Using a Two Enzyme Reactor
dc.typeOtro


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